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Rekombinanter Villin 1 Antikörper (AA 600-700)

VIL1 Reaktivität: Human IHC Wirt: Kaninchen Monoclonal MSVA-459R unconjugated Recombinant Antibody
Produktnummer ABIN7477617
  • Target Alle Villin 1 (VIL1) Antikörper anzeigen
    Villin 1 (VIL1)
    Antikörpertyp
    Recombinant Antibody
    Bindungsspezifität
    • 16
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 600-700
    Reaktivität
    • 74
    • 25
    • 19
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 47
    • 27
    Kaninchen
    Klonalität
    • 38
    • 36
    Monoklonal
    Konjugat
    • 46
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Villin 1 Antikörper ist unkonjugiert
    Applikation
    • 45
    • 26
    • 18
    • 17
    • 12
    • 8
    • 4
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC)
    Immunogen
    Recombinant fragment (around aa600-700) of human Villin protein (exact sequence is proprietary)
    Klon
    MSVA-459R
    Isotyp
    IgG
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  • Applikationshinweise
    IHC 1:100-1:200
    Kommentare

    Positive Control: Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen.

    Negative Control: Colon: Villin staining should be absent in all non-epithelial cells.

    Protokoll
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target
    Villin 1 (VIL1)
    Andere Bezeichnung
    Villin (VIL1 Produkte)
    Synonyme
    D2S1471 antikoerper, VIL antikoerper, Vil antikoerper, vil1 antikoerper, MGC64339 antikoerper, VIL1 antikoerper, vil1l antikoerper, zgc:55779 antikoerper, villin 1 antikoerper, villin 1 S homeolog antikoerper, VIL1 antikoerper, Vil1 antikoerper, vil1.S antikoerper, vil1 antikoerper
    Hintergrund
    VIL1, Villin-1, Villin1,Villin antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    P09327
    Pathways
    EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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