CAN Antikörper (AA 247-449)

Produktnummer ABIN967861

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CAN in WB, IHC, IP und IF. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Huhn und Frosch und wurde in 5+ Publikationen erwähnt.

Kurzübersicht für CAN Antikörper (AA 247-449) (ABIN967861)

Target: CAN (Calcineurin (CAN))

Reaktivität: Human, Maus, Ratte, Huhn, Frosch

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Klon: 29-Calcineurin

Produktdetails anti-CAN Antikörper

Bindungsspezifität: AA 247-449

Kreuzreaktivität: Ratte (Rattus), Maus, Huhn, Frosch

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Calcineurin aa. 247-449

Isotyp: IgG2a

Anwendungsinformationen

Kommentare:

Related Products: ABIN968545, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-CAN Antikörper (ABIN967861)

Vega, Rothermel, Weinheimer, Kovacs, Naseem, Bassel-Duby, Williams, Olson: "Dual roles of modulatory calcineurin-interacting protein 1 in cardiac hypertrophy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 669-74, (2003) (PubMed).

Jicha, Weaver, Lane, Vianna, Kress, Rockwood, Davies: "cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 17, pp. 7486-94, (1999) (PubMed).

Liang, Venema, Wang, Ju, Venema, Marrero: "Regulation of angiotensin II-induced phosphorylation of STAT3 in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19846-51, (1999) (PubMed).

Sík, Hájos, Gulácsi, Mody, Freund: "The absence of a major Ca2+ signaling pathway in GABAergic neurons of the hippocampus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 6, pp. 3245-50, (1998) (PubMed).

Verin, Cooke, Herenyiova, Patterson, Garcia: "Role of Ca2+/calmodulin-dependent phosphatase 2B in thrombin-induced endothelial cell contractile responses." in: The American journal of physiology, Vol. 275, Issue 4 Pt 1, pp. L788-99, (1998) (PubMed).

Details zu CAN

Target: CAN (Calcineurin (CAN))

Andere Bezeichnung: Calcineurin

Hintergrund: Calcineurin is a Ca2+/calmodulin-dependent protein phosphatase. The active enzyme is a heterodimer of a large calmodulin-binding catalytic subunit A (61 kDa) and a smaller Ca2+ binding subunit B (19 kDa). Regions corresponding to the calmodulin-binding site, an autoinhibitory domain, and a putative subunit B binding site have been identified within the large subunit A. The activity of calcineurin is sensitive to immunosuppressants such as cyclosporin A (CsA) and tacrolimus (FK506). The study of FK506-mediated inhibition of nitric oxide formation has revealed that nitric oxide synthase (NOS) is a calcineurin substrate. Calcineurin dephosphorylates NOS and enhances its catalytic activity. Therefore, Calcineurin is an essential mediator for efficient T cell antigen receptor (TCR)-mediated T cell activation.

Molekulargewicht: 61 kDa