PTGS2 Antikörper (AA 368-604)

Produktnummer ABIN967823

Der Maus Monoklonal Anti-PTGS2-Antikörper wurde für WB, IHC, IF und IP validiert. Er ist geeignet, PTGS2 in Proben von Human, Maus und Huhn zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für PTGS2 Antikörper (AA 368-604) (ABIN967823)

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Reaktivität: Human, Maus, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PTGS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 33-Cox

Produktdetails anti-PTGS2 Antikörper

Bindungsspezifität: AA 368-604

Kreuzreaktivität: Maus, Human, Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Cox-2 aa. 368-604

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968550, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20° C.

Referenzen für anti-PTGS2 Antikörper (ABIN967823)

Shiotani, Denda, Yamamoto, Kitayama, Endoh, Sasaki, Tsutsumi, Sugimura, Konishi: "Increased expression of cyclooxygenase-2 protein in 4-nitroquinoline-1-oxide-induced rat tongue carcinomas and chemopreventive efficacy of a specific inhibitor, nimesulide." in: Cancer research, Vol. 61, Issue 4, pp. 1451-6, (2001) (PubMed).

Giroux, Descoteaux: "Cyclooxygenase-2 expression in macrophages: modulation by protein kinase C-alpha." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3985-91, (2000) (PubMed).

Marrogi, Pass, Khan, Metheny-Barlow, Harris, Gerwin: "Human mesothelioma samples overexpress both cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (NOS2): in vitro antiproliferative effects of a COX-2 inhibitor." in: Cancer research, Vol. 60, Issue 14, pp. 3696-700, (2000) (PubMed).

Hla, Neilson: "Human cyclooxygenase-2 cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7384-8, (1992) (PubMed).

Xie, Cho, Calaycay, Mumford, Swiderek, Lee, Ding, Troso, Nathan: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, (1992) (PubMed).

Details zu PTGS2

Target: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Andere Bezeichnung: Cox-2

Hintergrund: Cyclooxygenase (Cox) is also known as prostaglandin H synthase or PGH synthase (E.C. 1.14.99.1). It catalyzes the conversion of arachidonate to prostaglandin H2 (PGH2), the precursor of PGE2, PGF2alpha, PGD2, prostacyclin, and thromboxane A2. Cox actually has two different enzymatic activities: a cyclooxygenase that mediates the formation of PGG2 from oxygen and arachidonate and a hydroperoxidase that catalyzes a reduction of PGG2 yielding PGH2. Two Cox genes, Cox-1 and Cox-2, have been isolated in several species. A 4kb mRNA encodes the 604 amino acid Cox-2 protein. The two human Cox isoenzymes are 61% identical in amino acid composition with the active sites being highly conserved. Cox-2 mRNA and protein levels are induced by serum, lipopolysaccharides, growth factors, human chorionic gonadotropin and phorbol testers in various mammalian cell types. It has been shown that interleukin-1alpha (IL-1alpha) induces increased levels of Cox-2 mRNA and protein in human endothelial cells. The sustained increase in Cox-2 is apparently due (at least in part) to IL-1alpha increasing the stability of Cox-2 mRNA. This type of regulatory mechanism may play an important role in chronic inflammatory conditions.
Synonyms: PGHS-2, Cyclooxygenase-2

Molekulargewicht: 70 kDa

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation