ErbB2/Her2 Antikörper (AA 182-373)

Produktnummer ABIN967787

Der Maus Monoklonal Anti-ErbB2/Her2-Antikörper wurde für WB, IHC, IF und IP validiert. Er ist geeignet, ErbB2/Her2 in Proben von Human zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für ErbB2/Her2 Antikörper (AA 182-373) (ABIN967787)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ErbB2/Her2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: 42-c-erbB

Produktdetails anti-ErbB2/Her2 Antikörper

Bindungsspezifität: AA 182-373

Kreuzreaktivität: Human

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat ErbB2 aa. 182-373

Isotyp: IgG2b

Anwendungsinformationen

Kommentare:

Related Products: ABIN968533, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-ErbB2/Her2 Antikörper (ABIN967787)

Dillon, Creer, Kerr, Kümin, Dickson: "Basolateral targeting of ERBB2 is dependent on a novel bipartite juxtamembrane sorting signal but independent of the C-terminal ERBIN-binding domain." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6553-63, (2002) (PubMed).

Piechocki, Pilon, Wei: "Complementary antitumor immunity induced by plasmid DNA encoding secreted and cytoplasmic human ErbB-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 6, pp. 3367-74, (2001) (PubMed).

Xu, Mimnaugh, Rosser, Nicchitta, Marcu, Yarden, Neckers: "Sensitivity of mature Erbb2 to geldanamycin is conferred by its kinase domain and is mediated by the chaperone protein Hsp90." in: The Journal of biological chemistry, Vol. 276, Issue 5, pp. 3702-8, (2001) (PubMed).

Graus-Porta, Beerli, Hynes: "Single-chain antibody-mediated intracellular retention of ErbB-2 impairs Neu differentiation factor and epidermal growth factor signaling." in: Molecular and cellular biology, Vol. 15, Issue 3, pp. 1182-91, (1995) (PubMed).

Szöllösi, Balázs, Feuerstein, Benz, Waldman: "ERBB-2 (HER2/neu) gene copy number, p185HER-2 overexpression, and intratumor heterogeneity in human breast cancer." in: Cancer research, Vol. 55, Issue 22, pp. 5400-7, (1995) (PubMed).

Details zu ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Andere Bezeichnung: c-erbB-2

Hintergrund: ErbB2 (Neu or Her-2) is a member of the erbB family of growth factor receptors. These factors possess constitutive tyrosine kinase activity and are commonly overexpressed in breast and ovarian carcinomas. While other erbB family members' ligands, such a EGF and NDF, are well characterized, a natural ligand for erbB2 remains unknown. ErbB2 forms heterodimers with erbB1/EGFR, erbB3, and erbB4, and can modulate their ligand affinities. Thus, erbB2 alters the intracellular responses elicited by EGF and NDF. This control is due to the fact that erbB2, when in complex with another erbB family receptor, decelerates the rate of ligand dissociation. Therefore, erbB2 may act as a signaling subunit for other receptors rather than a true growth factor receptor. Due to high sequence homology, this antibody may cross-react with the 180 kDa EGFR.
Synonyms: Neu, Her-2

Molekulargewicht: 185 kDa

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Skeletal Muscle Fiber Development