Cortactin Antikörper

Produktnummer ABIN967709

Der Maus Monoklonal Anti-Cortactin-Antikörper wurde für WB, IP, IF und IHC (f) validiert. Er ist geeignet, Cortactin in Proben von Human und Hund zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für Cortactin Antikörper (ABIN967709)

Target: Cortactin (CTTN)

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cortactin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 30-Cortactin

Produktdetails anti-Cortactin Antikörper

Kreuzreaktivität: Human, Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Chicken Cortactin

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN968533, ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-Cortactin Antikörper (ABIN967709)

Lavoie, Champagne, Gingras, Robert: "Adenovirus E4 open reading frame 4-induced apoptosis involves dysregulation of Src family kinases." in: The Journal of cell biology, Vol. 150, Issue 5, pp. 1037-56, (2000) (PubMed).

Nakahara, Mueller, Nomizu, Yamada, Yeh, Chen: "Activation of beta1 integrin signaling stimulates tyrosine phosphorylation of p190RhoGAP and membrane-protrusive activities at invadopodia." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 9-12, (1998) (PubMed).

Dehio, Prévost, Sansonetti: "Invasion of epithelial cells by Shigella flexneri induces tyrosine phosphorylation of cortactin by a pp60c-src-mediated signalling pathway." in: The EMBO journal, Vol. 14, Issue 11, pp. 2471-82, (1995) (PubMed).

Wu, Parsons: "Cortactin, an 80/85-kilodalton pp60src substrate, is a filamentous actin-binding protein enriched in the cell cortex." in: The Journal of cell biology, Vol. 120, Issue 6, pp. 1417-26, (1993) (PubMed).

Wu, Reynolds, Kanner, Vines, Parsons: "Identification and characterization of a novel cytoskeleton-associated pp60src substrate." in: Molecular and cellular biology, Vol. 11, Issue 10, pp. 5113-24, (1991) (PubMed).

Details zu Cortactin

Target: Cortactin (CTTN)

Andere Bezeichnung: Cortactin

Hintergrund: Cortactin is a protein which is represented by two structurally related isoforms of 80 kDa and 85 kDa. Sequence analysis of this protein reveals the presence of a carboxy-terminal SH3 domain, as well as six internal tandem repeats of a 37 amino acid sequence. It is able to bind to F-actin via this N-terminal repeat region, and colocalizes with actin in the cell. Cortactin was originally described as a protein that is tyrosine-phosphorylated in cells transformed by the src oncogene, in which it localizes to rosettes or podosomes. It has since been shown that other stimuli, such as bacterial invasion, treatment with growth factors, and integrin-mediated cell adhesion, are also capable of inducing phosphorylation of cortactin. Some additional evidence indicates that cortactin may actually be a substrate of syk, with src acting as an upstream signaling molecule. This antibody is routinely tested by western blot analysis.

Molekulargewicht: 85/80 kDa

Pathways: MAPK Signalweg