EGFR Antikörper (pTyr845)
-
- Target Alle EGFR Antikörper anzeigen
- EGFR (Epidermal Growth Factor Receptor (EGFR))
-
Bindungsspezifität
- pTyr845
-
Reaktivität
- Human, Maus, Ratte
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser EGFR Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Marke
- BD Pharmingen™
- Kreuzreaktivität
- Maus, Ratte (Rattus)
- Produktmerkmale
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols. - Aufreinigung
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Phosphorylated Human EGF Receptor Peptide
- Klon
- 12A3
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product EGFR Primärantikörper
-
-
- Kommentare
-
Related Products: ABIN967389
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store undiluted at 4°C.
-
-
A simple multicolor flow cytometry protocol for detection and molecular characterization of circulating tumor cells in epithelial cancers." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 81, Issue 6, pp. 489-95, (2012) (PubMed).
: "Phosphorylation of Y845 on the epidermal growth factor receptor mediates binding to the mitochondrial protein cytochrome c oxidase subunit II." in: Molecular and cellular biology, Vol. 24, Issue 16, pp. 7059-71, (2004) (PubMed).
: "Status of epidermal growth factor receptor antagonists in the biology and treatment of cancer." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 21, Issue 14, pp. 2787-99, (2003) (PubMed).
: "Integrin-induced epidermal growth factor (EGF) receptor activation requires c-Src and p130Cas and leads to phosphorylation of specific EGF receptor tyrosines." in: The Journal of biological chemistry, Vol. 277, Issue 11, pp. 9405-14, (2002) (PubMed).
: "Src-dependent phosphorylation of the epidermal growth factor receptor on tyrosine 845 is required for zinc-induced Ras activation." in: The Journal of biological chemistry, Vol. 277, Issue 27, pp. 24252-7, (2002) (PubMed).
: "The ErbB signaling network: receptor heterodimerization in development and cancer." in: The EMBO journal, Vol. 19, Issue 13, pp. 3159-67, (2000) (PubMed).
: "c-Src-mediated phosphorylation of the epidermal growth factor receptor on Tyr845 and Tyr1101 is associated with modulation of receptor function." in: The Journal of biological chemistry, Vol. 274, Issue 12, pp. 8335-43, (1999) (PubMed).
: "
-
A simple multicolor flow cytometry protocol for detection and molecular characterization of circulating tumor cells in epithelial cancers." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 81, Issue 6, pp. 489-95, (2012) (PubMed).
-
- Target
- EGFR (Epidermal Growth Factor Receptor (EGFR))
- Andere Bezeichnung
- EGF Receptor (EGFR Produkte)
- Hintergrund
-
Epidermal Growth Factor (EGF) elicits a variety of cellular responses that are initiated by EGF Receptor (EGFR) binding and activation of intrinsic tyrosine kinase activity. EGFR, also known as ErbB1 or HER1, is a member of the ErbB class of receptor protein tyrosine kinases. It has an extracellular ligand-binding domain, a single transmembrane region, and a cytoplasmic region containing a protein tyrosine kinase domain and a c-terminal regulatory domain with many phosphorylation sites. Following ligand binding, EGFR forms homodimers and heterodimers with ErbB2. Specific C-terminal tyrosine residues are then autophosphorylated and, in turn, bind to adaptor proteins, kinases, or protein tyrosine phosphatases. In addition, c-Src-dependent phosphorylations at other sites, such as tyrosine 845 (Y845) in the kinase domain, regulate the receptor's kinase activity. Inappropriate expression or mutations of EGFR and/or deregulation of its signaling pathways are associated with many types of cancer, making EGFR a promising target for cancer therapies.
The 12A3 monoclonal antibody recognizes the phosphorylated Y845 in the protein tyrosine kinase domain of human EGFR. - Molekulargewicht
- 175 kDa
- Pathways
- NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine
-