B-Cell Linker Antikörper (pTyr84)

Produktnummer ABIN967596

Dieses Anti-B-Cell Linker-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von B-Cell Linker in WB. Geeignet für Human und Maus. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für B-Cell Linker Antikörper (pTyr84) (ABIN967596)

Target: B-Cell Linker (BLNK)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser B-Cell Linker Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: J117-1278

Produktdetails anti-B-Cell Linker Antikörper

Bindungsspezifität: pTyr84

Marke: BD Pharmingen™

Kreuzreaktivität: Maus

Produktmerkmale: 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human BLNK Peptide

Isotyp: IgG2b kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-B-Cell Linker Antikörper (ABIN967596)

Pighi, Gu, Dalai, Barbi, Parolini, Bertolaso, Pedron, Parisi, Ren, Cecconi, Chilosi, Menestrina, Zamò: "Phospho-proteomic analysis of mantle cell lymphoma cells suggests a pro-survival role of B-cell receptor signaling." in: Cellular oncology (Dordrecht), Vol. 34, Issue 2, pp. 141-53, (2011) (PubMed).

Espeli, Mancini, Breton, Poirier, Schiff: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." in: Blood, Vol. 113, Issue 23, pp. 5878-86, (2009) (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, (2003) (PubMed).

Chiu, Dalton, Ishiai, Kurosaki, Chan: "BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins." in: The EMBO journal, Vol. 21, Issue 23, pp. 6461-72, (2002) (PubMed).

Details zu B-Cell Linker

Target: B-Cell Linker (BLNK)

Andere Bezeichnung: BLNK

Hintergrund: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.

Molekulargewicht: 68 kDa

Pathways: BCR Signaling