CD24 Antikörper

Produktnummer ABIN967572

Dieses Anti-CD24-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von CD24 in FACS, WB, IHC (fro), CyTox, IHC (zinc) und IHC (f). Geeignet für Maus. Dieses Primary Antibody wurde in 14+ Publikationen zitiert.

Kurzübersicht für CD24 Antikörper (ABIN967572)

Target: CD24 (CD24 Molecule (CD24))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD24 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: M1-69

Produktdetails anti-CD24 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE™ (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C57BL/10 Mouse Splenic T Lymphocytes

Isotyp: IgG2b kappa

Anwendungsinformationen

Kommentare:

Related Products: ABIN967372

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Referenzen für anti-CD24 Antikörper (ABIN967572)

Hunte, Capone, Zlotnik, Rennick, Moore: "Acquisition of CD24 expression by Lin-CD43+B220(low)ckit(hi) cells coincides with commitment to the B cell lineage." in: European journal of immunology, Vol. 28, Issue 11, pp. 3850-6, (1998) (PubMed).

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).

Calaora, Chazal, Nielsen, Rougon, Moreau: "mCD24 expression in the developing mouse brain and in zones of secondary neurogenesis in the adult." in: Neuroscience, Vol. 73, Issue 2, pp. 581-94, (1997) (PubMed).

Aigner, Ruppert, Hubbe, Sammar, Sthoeger, Butcher, Vestweber, Altevogt: "Heat stable antigen (mouse CD24) supports myeloid cell binding to endothelial and platelet P-selectin." in: International immunology, Vol. 7, Issue 10, pp. 1557-65, (1996) (PubMed).

Auerbach, Huang, Lu: "Hematopoietic stem cells in the mouse embryonic yolk sac." in: Stem cells (Dayton, Ohio), Vol. 14, Issue 3, pp. 269-80, (1996) (PubMed).

Kennedy, Mohler, Shanebeck, Baum, Picha, Otten-Evans, Janeway, Grabstein: "Induction of B cell costimulatory function by recombinant murine CD40 ligand." in: European journal of immunology, Vol. 24, Issue 1, pp. 116-23, (1994) (PubMed).

Kelly, Pearse, Lefrancois, Scollay: "Emigration of selected subsets of gamma delta + T cells from the adult murine thymus." in: International immunology, Vol. 5, Issue 4, pp. 331-5, (1993) (PubMed).

Wenger, Rochelle, Seldin, Köhler, Nielsen: "The heat stable antigen (mouse CD24) gene is differentially regulated but has a housekeeping promoter." in: The Journal of biological chemistry, Vol. 268, Issue 31, pp. 23345-52, (1993) (PubMed).

Vremec, Zorbas, Scollay, Saunders, Ardavin, Wu, Shortman: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, (1992) (PubMed).

Alterman, Crispe, Kinnon: "Characterization of the murine heat-stable antigen: an hematolymphoid differentiation antigen defined by the J11d, M1/69 and B2A2 antibodies." in: European journal of immunology, Vol. 20, Issue 7, pp. 1597-602, (1990) (PubMed).

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, (1989) (PubMed).

Veillette, Zúñiga-Pflücker, Bolen, Kruisbeek: "Engagement of CD4 and CD8 expressed on immature thymocytes induces activation of intracellular tyrosine phosphorylation pathways." in: The Journal of experimental medicine, Vol. 170, Issue 5, pp. 1671-80, (1989) (PubMed).

Wilson, Day, Scollay, Shortman: "Subpopulations of mature murine thymocytes: properties of CD4-CD8+ and CD4+CD8- thymocytes lacking the heat-stable antigen." in: Cellular immunology, Vol. 117, Issue 2, pp. 312-26, (1989) (PubMed).

Bruce, Symington, McKearn, Sprent: "A monoclonal antibody discriminating between subsets of T and B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2496-501, (1982) (PubMed).

Details zu CD24

Target: CD24 (CD24 Molecule (CD24))

Andere Bezeichnung: CD24

Hintergrund: The M1/69 antibody reacts with CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated GPI-anchored membrane protein found on erythrocytes, granulocytes, monocytes, lymphocytes, and neurons. Hematopoietic stem cells of the embryonic yolk sac and fetal liver express CD24.5 Levels of expression of CD24 vary during differentiation of the T and B cell lineages. In the bone marrow, hematopoietic progenitors acquire CD24 expression upon commitment to the B-lymphocyte lineage. Immature B cells in the bone marrow and spleen of adult mice peripheral B lymphocytes express intermediate levels of CD24. The level of CD24 expression has been reported to rise upon activation of splenic B cells with LPS, but not with CD154 (CD40 Ligand). The majority of thymocytes express high levels of CD24, while most mature thymic and peripheral T lymphocytes do not express CD24. In contrast, TCR-bearing thymocytes which emigrate to the spleen are CD24+. Dendritic cells of the thymus, spleen, liver, and epidermal Langerhans cells have also been reported to express CD24. CD24 is not expressed by NK cells, as determined by staining with J11d mAb (ABIN967372). CD24 is involved in the costimulation of CD4+ T cells by B cells, it is a co-inducer of in vitro thymocyte maturation, and it is a ligand of CD62P (P-selectin). While the monoclonal antibodies 30-F1, M1/69, and J11d all react with CD24, they show subtle differences in the level of staining of different lymphocyte populations. When possible, investigators should continue to use the same monoclonal antibody as used in previous studies. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Heat Stable Antigen

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation