CD24 Antikörper (CD24 Molecule) (APC)

Details for Product anti-CD24 Antibody No. ABIN2749227
Antigen
  • CD24A
  • Cd24a
  • CD24 molecule
  • CD24
  • Cd24
Reaktivität
Human
176
167
28
4
1
Wirt
Maus
137
117
77
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD24 Antikörper ist konjugiert mit APC
48
43
28
16
6
5
5
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Microcytotoxicity Assay (MCA)
241
68
61
43
42
27
26
13
13
8
3
3
3
2
1
Optionen
Immunogen Glycoproteins purified from human NALM-1 cell line.
Klon SN3
Isotyp IgG1
Spezifität The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.
HLDA IV, WS Code B 136
HLDA V, WS Code B CD24.7
Kreuzreaktivität (Details) Human
Plasmids, Primers & others Plasmide, Primers & weitere CD24 products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung CD24 (CD24 Antibody Abstract)
Hintergrund CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4, it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.
Gen-ID 100133941
Pathways Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
Applikations-hinweise The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Kommentare

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Beschränkungen Nur für Forschungszwecke einsetzbar
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD24 Molecule (CD24) antibody (APC) (ABIN2749227) Surface staining of human peripheral blood cells with anti-CD24 (SN3) Alexa Fluor®...
Produkt verwendet in: Všianská, Říhová, Varmužová, Suská, Kryukov, Mikulášová, Kupská, Penka, Pour, Adam, Hájek: "Analysis of B-cell subpopulations in monoclonal gammopathies." in: Clinical lymphoma, myeloma & leukemia, Vol. 15, Issue 4, pp. e61-71, 2015 (PubMed).

Maliar, Servais, Waks, Chmielewski, Lavy, Altevogt, Abken, Eshhar: "Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice." in: Gastroenterology, Vol. 143, Issue 5, pp. 1375-84.e1-5, 2012 (PubMed).

Solvason, Kearney: "The human fetal omentum: a site of B cell generation." in: The Journal of experimental medicine, Vol. 175, Issue 2, pp. 397-404, 1992 (PubMed).

Fischer, Majdic, Gadd, Knapp: "Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 2, pp. 638-41, 1990 (PubMed).

Barcos, Pollard, Fukukawa, Seon: "Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3." in: Hematological oncology, Vol. 4, Issue 4, pp. 251-9, 1987 (PubMed).

Fukukawa, Matsuzaki, Haruta, Hara, Seon: "New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non-T leukemia cells." in: Experimental hematology, Vol. 14, Issue 9, pp. 850-5, 1986 (PubMed).

Allgemeine Veröffentlichungen Runz, Mierke, Joumaa, Behrens, Fabry, Altevogt: "CD24 induces localization of beta1 integrin to lipid raft domains." in: Biochemical and biophysical research communications, Vol. 365, Issue 1, pp. 35-41, 2007 (PubMed).

Chou, Jeng, Lee, Hu, Kao, Lin, Lai, Hu, Yuan: "Cytoplasmic CD24 expression is a novel prognostic factor in diffuse-type gastric adenocarcinoma." in: Annals of surgical oncology, Vol. 14, Issue 10, pp. 2748-58, 2007 (PubMed).

Keller, Rupp, Stoeck, Runz, Fogel, Lugert, Hager, Abdel-Bakky, Gutwein, Altevogt: "CD24 is a marker of exosomes secreted into urine and amniotic fluid." in: Kidney international, Vol. 72, Issue 9, pp. 1095-102, 2007 (PubMed).

Schabath, Runz, Joumaa, Altevogt: "CD24 affects CXCR4 function in pre-B lymphocytes and breast carcinoma cells." in: Journal of cell science, Vol. 119, Issue Pt 2, pp. 314-25, 2006 (PubMed).

Suzuki, Kiyokawa, Taguchi, Sekino, Katagiri, Fujimoto: "CD24 induces apoptosis in human B cells via the glycolipid-enriched membrane domains/rafts-mediated signaling system." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 9, pp. 5567-77, 2001 (PubMed).