P-Glycoprotein Antikörper

Produktnummer ABIN967562

Der Maus Monoklonal Anti-P-Glycoprotein-Antikörper wurde für WB, FACS und IP validiert. Er ist geeignet, P-Glycoprotein in Proben von Human zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für P-Glycoprotein Antikörper (ABIN967562)

Target: P-Glycoprotein

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser P-Glycoprotein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon: 17F9

Produktdetails anti-P-Glycoprotein Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Positive control cells are available through special order

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-P-Glycoprotein Antikörper (ABIN967562)

Shi, Wrin, Reeder, Liu, Ring: "High-affinity monoclonal antibodies against P-glycoprotein." in: Clinical immunology and immunopathology, Vol. 76, Issue 1 Pt 1, pp. 44-51, (1995) (PubMed).

Bénard, Bourhis, Riou: "Clinical significance of multiple drug resistance in human cancers." in: Anticancer research, Vol. 10, Issue 5A, pp. 1297-302, (1990) (PubMed).

Goldstein, Galski, Fojo, Willingham, Lai, Gazdar, Pirker, Green, Crist, Brodeur: "Expression of a multidrug resistance gene in human cancers." in: Journal of the National Cancer Institute, Vol. 81, Issue 2, pp. 116-24, (1989) (PubMed).

Details zu P-Glycoprotein

Target: P-Glycoprotein

Hintergrund: Reacts with a 170-180 kDa transmembrane glycoprotein (P-glycoprotein), a product of the multidrug resistance-1 (MDR1) gene. This glycoprotein is expressed on MDR positive cells and has been reported to be expressed on many normal tissues, such as adrenal glands and endothelium, in the brain and skin. P-glycoprotein is known to impart drug resistance to cells by pumping many anti-cancer drugs out of the cytoplasm. 17F9 antibody binds to the surface of the viable, unfixed MDR+ cell line, PMG-Y (please inquire), and is able to partially block the binding of UIC2 antibody (another MDR-specific monoclonal antibody). Immunoprecipitation application is reported, but not routinely tested in house.
Synonyms: MDR