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TUBB Antikörper

TUBB Reaktivität: Human, Maus, Ratte, Rind (Kuh) WB, IHC, BI Wirt: Maus Monoclonal 5H1 unconjugated
Produktnummer ABIN967495
  • Target Alle TUBB Antikörper anzeigen
    TUBB (Tubulin, beta (TUBB))
    Reaktivität
    • 173
    • 143
    • 133
    • 43
    • 29
    • 23
    • 19
    • 16
    • 15
    • 13
    • 12
    • 10
    • 8
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Rind (Kuh)
    Wirt
    • 110
    • 76
    • 1
    • 1
    • 1
    Maus
    Klonalität
    • 96
    • 89
    • 2
    Monoklonal
    Konjugat
    • 100
    • 9
    • 9
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Dieser TUBB Antikörper ist unkonjugiert
    Applikation
    • 168
    • 49
    • 42
    • 39
    • 37
    • 33
    • 29
    • 28
    • 18
    • 13
    • 11
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), BioImaging (BI)
    Marke
    BD Pharmingen™
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
    3. Triton is a trademark of the Dow Chemical Company.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. Please refer to us for technical protocols.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Klon
    5H1
    Isotyp
    IgM kappa
    Top Product
    Discover our top product TUBB Primärantikörper
  • Applikationshinweise
    Bioimaging
    1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
    2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
    3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
    4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
    5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
    6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
    7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
    8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
    9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
    10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
    11. View and analyze the cells on an appropriate imaging instrument.
    Kommentare

    Related Products: ABIN967389

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store undiluted at 4°C.
  • Cho, Johnson: "Primed phosphorylation of tau at Thr231 by glycogen synthase kinase 3beta (GSK3beta) plays a critical role in regulating tau's ability to bind and stabilize microtubules." in: Journal of neurochemistry, Vol. 88, Issue 2, pp. 349-58, (2003) (PubMed).

    Helfand, Mikami, Vallee, Goldman: "A requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization." in: The Journal of cell biology, Vol. 157, Issue 5, pp. 795-806, (2002) (PubMed).

    Prahlad, Yoon, Moir, Vale, Goldman: "Rapid movements of vimentin on microtubule tracks: kinesin-dependent assembly of intermediate filament networks." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 159-70, (1998) (PubMed).

    Brown, Wood, Cleveland: "The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A." in: Journal of cell science, Vol. 109 ( Pt 5), Issue 4, pp. 961-9, (1996) (PubMed).

    Wang, Loomis, Zinkowski, Binder: "A novel tau transcript in cultured human neuroblastoma cells expressing nuclear tau." in: The Journal of cell biology, Vol. 121, Issue 2, pp. 257-67, (1993) (PubMed).

  • Target
    TUBB (Tubulin, beta (TUBB))
    Andere Bezeichnung
    beta-Tubulin (TUBB Produkte)
    Synonyme
    M40 antikoerper, OK/SW-cl.56 antikoerper, TUBB1 antikoerper, TUBB5 antikoerper, XLOT antikoerper, m40 antikoerper, ok/sw-cl.56 antikoerper, tubb1 antikoerper, tubb5 antikoerper, TUBB2A antikoerper, TUBB2B antikoerper, TUBB antikoerper, 1t antikoerper, B1t antikoerper, BETA 56D antikoerper, CG9277 antikoerper, DTB2 antikoerper, Dmbeta1 antikoerper, Dmel\\CG9277 antikoerper, T antikoerper, Tub antikoerper, Tubulin antikoerper, beta antikoerper, beta-Tub antikoerper, beta-Tub56D antikoerper, beta-tub antikoerper, beta-tubulin56D antikoerper, beta1 antikoerper, beta1-Tubulin antikoerper, beta1-tub antikoerper, beta1Tub antikoerper, beta1t antikoerper, beta1tub antikoerper, beta56D antikoerper, betaTub antikoerper, betaTub1 antikoerper, beta[[1]] tubulin antikoerper, beta[[1]]-tubulin antikoerper, betatub(56D) antikoerper, 143391_i_at antikoerper, 3t antikoerper, B3t antikoerper, BETA 60D antikoerper, CG3401 antikoerper, D.m.BETA-60D antikoerper, DTB3 antikoerper, Dmbeta3 antikoerper, Dmel\\CG3401 antikoerper, Tub60D antikoerper, beta-Tub60D antikoerper, beta-Tub6D antikoerper, beta3 antikoerper, beta3 TU antikoerper, beta3-Tub antikoerper, beta3-tubulin antikoerper, beta3TUB antikoerper, beta3Tub antikoerper, beta3t antikoerper, beta60C antikoerper, betaTub3 antikoerper, betaTub60C antikoerper, beta[[3]] tubulin antikoerper, beta[[3]]-Tub antikoerper, beta[[3]]-tubulin antikoerper, betatub60D antikoerper, p50 antikoerper, p50/tubulin antikoerper, p53 antikoerper, p53/tubulin antikoerper, 2t antikoerper, B2t antikoerper, BETA 85D antikoerper, BETA2 antikoerper, CG9359 antikoerper, D.m.BETA-85D antikoerper, DTB4 antikoerper, Dmbeta2 antikoerper, Dmel\\CG9359 antikoerper, beta(2)Tu antikoerper, beta(2)Tub antikoerper, beta-Tub85D antikoerper, beta-tub85D antikoerper, beta2 antikoerper, beta2-tub antikoerper, beta2t antikoerper, beta2tub antikoerper, beta85D antikoerper, betaTub2 antikoerper, beta[[2]]-tubulin antikoerper, ms(3)KK[D] antikoerper, 4t antikoerper, B4t antikoerper, BETA 98B antikoerper, CG4869 antikoerper, DTB1 antikoerper, Dmbeta4 antikoerper, Dmel\\CG4869 antikoerper, beta-Tub97EF antikoerper, beta4 antikoerper, beta4t antikoerper, beta97F antikoerper, betaTub4 antikoerper, betaTub98BC antikoerper, betaTub98C antikoerper, Tub1 antikoerper, bmtub1 antikoerper, Tub3 antikoerper, bmtub3 antikoerper, Tub2 antikoerper, bmtub2 antikoerper, Tub4 antikoerper, bmtub4 antikoerper, LOC100101153 antikoerper, tubulin beta class I antikoerper, putative cobalt-zinc-cadmium outer membrane resistance protein antikoerper, Tubulin beta chain antikoerper, tubulin beta-2A chain antikoerper, beta-Tubulin at 56D antikoerper, beta-Tubulin at 60D antikoerper, beta-Tubulin at 85D antikoerper, beta-Tubulin at 97EF antikoerper, beta-tubulin antikoerper, tubulin beta 6 class V antikoerper, beta tubulin antikoerper, tubulin beta class I L homeolog antikoerper, tubulin, beta 5 class I antikoerper, TUBB antikoerper, czcC antikoerper, tbb-6 antikoerper, tubb antikoerper, LOC462399 antikoerper, betaTub56D antikoerper, betaTub60D antikoerper, betaTub85D antikoerper, betaTub97EF antikoerper, Tub1 antikoerper, Tub3 antikoerper, Tub2 antikoerper, Tub4 antikoerper, TVAG_525430 antikoerper, TVAG_062880 antikoerper, TVAG_456920 antikoerper, LOC100101153 antikoerper, TUBB6 antikoerper, Tb927.1.2330 antikoerper, Tb927.1.2350 antikoerper, Tb927.1.2370 antikoerper, Tb927.1.2390 antikoerper, tubb.L antikoerper, Tubb antikoerper, LOC693049 antikoerper, LOC443015 antikoerper, Tubb5 antikoerper
    Hintergrund
    Tubulin is a highly conserved protein with a molecular weight of ~50 kD. The self-assembly of tubulin leads to microtubules, hollow cylinders that are one of the major components of the eukaryotic cytoskeleton. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. There are two main classes of tubulin isoforms, alpha- and beta-tubulin, which are usually products of separate genes. Microtubules are made from protofilaments, strings of alternating alpha- and beta-tubulin spaced 4 nm apart and pointing in the same direction. Tubulin can be posttranslationally modified in several ways, including phosphorylation, acetylation, glutamylation, and detyrosination. For example, microtubules that turn over slowly tend to be acetylated and detyrosinated.
    The 5H1 monoclonal antibody reacts with beta-tubulin. It does not cross-react with alpha-tubulin.
    Molekulargewicht
    50 kDa
    Pathways
    Microtubule Dynamics, M Phase
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