SPI1 Antikörper

Produktnummer ABIN967452

Dieses Anti-SPI1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SPI1 in WB und IP. Geeignet für Human. Dieses Primary Antibody wurde in 5+ Publikationen zitiert.

Kurzübersicht für SPI1 Antikörper (ABIN967452)

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SPI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Klon: G148-74

Produktdetails anti-SPI1 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: PU.1 Fusion Protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Applications include gel shift, western blot analysis (1-2 µg/ml) and immunoprecipitation. The antibody has been shown to recognize in vitro translated PU.1 and PU.1 expressed as a recombinant protein in bacteria. In. In gel shift assays using µB as a probe (µB is a transcriptional element in the immunoglobulin heavy chain enhancer), G148-74 supershifts the complex of probe and PU.1.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-SPI1 Antikörper (ABIN967452)

Klemsz, McKercher, Celada, Van Beveren, Maki: "Pillars article: the macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell, 1990. 61: 113-124." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 3, pp. 1597-608, (2008) (PubMed).

Rivera, Stuiver, Steenbergen, Murre: "Ets proteins: new factors that regulate immunoglobulin heavy-chain gene expression." in: Molecular and cellular biology, Vol. 13, Issue 11, pp. 7163-9, (1993) (PubMed).

Shin, Koshland: "Ets-related protein PU.1 regulates expression of the immunoglobulin J-chain gene through a novel Ets-binding element." in: Genes & development, Vol. 7, Issue 10, pp. 2006-15, (1993) (PubMed).

Pongubala, Nagulapalli, Klemsz, McKercher, Maki, Atchison: "PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity." in: Molecular and cellular biology, Vol. 12, Issue 1, pp. 368-78, (1992) (PubMed).

Moreau-Gachelin, Ray, Mattei, Tambourin, Tavitian: "The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias." in: Oncogene, Vol. 4, Issue 12, pp. 1449-56, (1990) (PubMed).

Details zu SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Andere Bezeichnung: PU.1

Hintergrund: PU.1 is an ets-related transcription factor expressed in B lymphocytes and macrophages. It was initially identified as the oncogene Spi-1 which was found to block erythroblast differentiation. PU.1 functions by binding transcriptional control elements such as µB in the immunoglobulin heavy chain enhancer and pi adjacent to µE2. It has been shown to form a complex with NF-EM5, a B cell transcription factor, resulting in the activation of the immunoglobulin kappa 3' enhancer. PU.1 also appears to be critically involved in the control of monocyte development by regulating the expression of the macrophage colony-stimulating factor receptor. PU.1 runs as a 40-42 kDa protein on SDS-PAGE. G148-74 recognizes human PU.1, an Ets-related transcription factor. PU.1 is expressed in B lymphocytes and macrophages and like other Ets-related proteins, binds to consensus sites in DNA through an 85 amino acid Ets domain in the carboxyl terminal region of the protein. The antibody was raised against a bacterially expressed GST-PU.1 fusion protein.

Molekulargewicht: 40-42 kDa

Pathways: Stem Cell Maintenance