ERBB3 Antikörper

Produktnummer ABIN967437

Dieses Anti-ERBB3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ERBB3 in WB, IP, IHC (fro) und IHC (f). Geeignet für Human. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für ERBB3 Antikörper (ABIN967437)

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERBB3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: RTJ-1

Produktdetails anti-ERBB3 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human c-erbB-3 Peptide

Isotyp: IgM

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-ERBB3 Antikörper (ABIN967437)

Carraway, Cantley: "A neu acquaintance for erbB3 and erbB4: a role for receptor heterodimerization in growth signaling." in: Cell, Vol. 78, Issue 1, pp. 5-8, (1994) (PubMed).

Rajkumar, Gooden, Lemoine, Gullick, Goden: "Expression of the c-erbB-3 protein in gastrointestinal tract tumours determined by monoclonal antibody RTJ1." in: The Journal of pathology, Vol. 170, Issue 3, pp. 271-8, (1994) (PubMed).

Sanidas, Filipe, Linehan, Lemoine, Gullick, Rajkumar, Levison: "Expression of the c-erbB-3 gene product in gastric cancer." in: International journal of cancer. Journal international du cancer, Vol. 54, Issue 6, pp. 935-40, (1993) (PubMed).

Prigent, Lemoine, Hughes, Plowman, Selden, Gullick: "Expression of the c-erbB-3 protein in normal human adult and fetal tissues." in: Oncogene, Vol. 7, Issue 7, pp. 1273-8, (1992) (PubMed).

Details zu ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Andere Bezeichnung: c-erbB-3

Hintergrund: C-erbB-3, a glycoprotein of 160 kD, is a member of the type 1 growth factor receptor subfamily which also includes c-erbB-2 (HER2/neu), c-erbB-4 and the epidermal growth factor receptor (EGFR, c-erbB-1). Members of this receptor subfamily mediate the proliferation and differentiation of normal cells. They have a common structure consisting of an extracellular domain, a transmembrane region, and a cytoplasmic sequence. The extracellular regions contain two cysteine-rich domains, and the intracellular regions have sequence homology to known tyrosine kinases. C-erbB-3 is expressed in tissues from the digestive, urinary and respiratory tracts, the circulatory system, and female and male reproductive organs. c-erb B-3 is undetectable in hematopoietic tissue and cell lines derived from hematopoietic tumors. Cellular localization has been described as cytoplasmic and/or membrane, and nuclear. The level and pattern of c-erbB-3 expression varies widely in both normal and tumor tissues.
Clone RTJ.1 recognizes an epitope in the cytoplasmic domain of the human c-erbB-3 protein. It does not react with the EGF receptor or c-erbB-2. A synthetic peptide (referred to as 49.3) from the cytoplasmic domain of human c-erbB-3 protein was used as immunogen. RTJ.1 identifies a 160 kD band corresponding to c-erbB-3 by western blot analysis and immunoprecipitation. RTJ.1 may also react with two additional, unidentified higher molecular weight bands by immunoprecipitation and western blot analysis. These bands are likely to be non-specific, as they were not detected with a polyclonal antibody raised against the same immunogen. Because two additional bands were observed, the specificity of RTJ.1 for immunohistochemistry was analyzed by comparing staining results to those obtained using three polyclonal antibodies also raised against 49.3. Identical staining results were obtained using RTJ.1 and all three polyclonal antibodies. These results validated the use of RTJ.1 for immunohistochemical analysis of c-erbB-3.

Molekulargewicht: 160 kDa

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung