MYOD1 Antikörper

Produktnummer ABIN967403

Der Maus Monoklonal Anti-MYOD1-Antikörper wurde für WB und IHC (fro) validiert. Er ist geeignet, MYOD1 in Proben von Human, Maus, Ratte und Huhn zu detektieren. Es sind 5+ Publikationen verfügbar.

Kurzübersicht für MYOD1 Antikörper (ABIN967403)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: 5-8A

Produktdetails anti-MYOD1 Antikörper

Marke: BD Pharmingen™

Kreuzreaktivität: Human, Ratte (Rattus), Huhn

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Mouse MyoD

Isotyp: IgG1 kappa

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Referenzen für anti-MYOD1 Antikörper (ABIN967403)

Rawls, Valdez, Zhang, Richardson, Klein, Olson: "Overlapping functions of the myogenic bHLH genes MRF4 and MyoD revealed in double mutant mice." in: Development (Cambridge, England), Vol. 125, Issue 13, pp. 2349-58, (1998) (PubMed).

Gredinger, Gerber, Tamir, Tapscott, Bengal: "Mitogen-activated protein kinase pathway is involved in the differentiation of muscle cells." in: The Journal of biological chemistry, Vol. 273, Issue 17, pp. 10436-44, (1998) (PubMed).

Dias, Dilling, Houghton: "The molecular basis of skeletal muscle differentiation." in: Seminars in diagnostic pathology, Vol. 11, Issue 1, pp. 3-14, (1994) (PubMed).

Dias, Parham, Shapiro, Tapscott, Houghton: "Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility." in: Cancer research, Vol. 52, Issue 23, pp. 6431-9, (1992) (PubMed).

Davis, Weintraub, Lassar: "Expression of a single transfected cDNA converts fibroblasts to myoblasts." in: Cell, Vol. 51, Issue 6, pp. 987-1000, (1988) (PubMed).

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MyoD

Hintergrund: MyoD is a member of a family of myogenic basic helix-loop-helix (bHLH) transcription factors that includes Myf-5, myogenin, and MRF4 (also known as herculin or Myf-6). Members of this family, expressed exclusively in skeletal muscle, play a key role in myogenesis by activating muscle-specific genes in response to extracellular growth factors. Transfection of MyoD and other family members into non-muscle cells has been shown to either convert these cells to myogenic cells or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. Members of this family have distinct roles in muscle development, for example, MyoD and Myf-5 act early during myeloid development, whereas myogenin acts at a later point during myoblast differentiation. The expression and activity of MyoD has been shown to involve the mitogen-activated protein kinase (MAPK) cascade. This antibody is routinely tested by western blot analysis. The epitope for MoAb 5.8A has been mapped to amino acids 180-189nof mouse MyoD.

Molekulargewicht: 45/48 kDa

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development