Rekombinanter MYOD1 Antikörper

Produktnummer ABIN6940161

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch MYOD1 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Meerschweinchen, Hamster und Kaninchen.

Kurzübersicht für Rekombinanter MYOD1 Antikörper (ABIN6940161)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte, Meerschweinchen, Hamster, Kaninchen

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MYOD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: MYOD1-2075R

Produktdetails anti-MYOD1 Antikörper

Verwendungszweck: Recombinant MyoD1 (Rhabdomyosarcoma Marker) Antibody

Spezifität: Recognizes a phospho-protein of 45 kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.

Aufreinigung: 200ug/ml of Ab purified by Protein A Column.

Immunogen: Recombinant human full-length MyoD1 protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Fetal skeletal muscle or Rhabdomyosarcoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Andere Bezeichnung: MYOD1

Hintergrund:

bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Human skeletal muscle or rhabdomyosarcoma, Heart

Molekulargewicht: 45kDa

Gen-ID: 4654, 181768

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development