LAMP1 Antikörper

Produktnummer ABIN967382

Der Ratte Monoklonal Anti-LAMP1-Antikörper wurde für WB, IP, IHC (fro), ICS und IAC validiert. Er ist geeignet, LAMP1 in Proben von Maus zu detektieren. Es sind 4+ Publikationen verfügbar.

Kurzübersicht für LAMP1 Antikörper (ABIN967382)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LAMP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunoaffinity Chromatography (IAC)

Klon: 1D4B

Produktdetails anti-LAMP1 Antikörper

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Plasma membrane fraction of mouse embryo NIH 3T3 cell line

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: This antibody conjugate has been tested by intracellular immunofluorescent staining ( Less or equal than 1 µg/million cells, using the Cytofix/Cytoperm™ Kit) with flow cytometric analysis to assure specificity and reactivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C.

Referenzen für anti-LAMP1 Antikörper (ABIN967382)

Rohrer, Schweizer, Russell, Kornfeld: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, (1996) (PubMed).

Arterburn, Earles, August: "The disulfide structure of mouse lysosome-associated membrane protein 1." in: The Journal of biological chemistry, Vol. 265, Issue 13, pp. 7419-23, (1990) (PubMed).

Chen, Chen, DSouza, Murphy, August: "Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2." in: Biochemical Society symposium, Vol. 51, pp. 97-112, (1987) (PubMed).

Chen, Pan, DSouza, August: "Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells." in: Archives of biochemistry and biophysics, Vol. 239, Issue 2, pp. 574-86, (1985) (PubMed).

Details zu LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Andere Bezeichnung: CD107a

Hintergrund: The 1D4B antibody recognizes CD107a (Lysosome-Associated Membrane Protein 1, LAMP-1), one of the two major glycoproteins in lysosome membranes which are useful markers to distinguish lysosomes from other organelles. Mouse CD107a consists of a 40-kDa core protein which is heavily glycosylated to form heterogeneous mature glycoprotein of 110-140 kDa. It is principally expressed in epithelial cells and macrophages in a variety of organs in normal and Beige mutant mice. Several lines of evidence suggest that cell-surface mouse and human LAMP-1 glycoproteins, the expression of which may correlate with malignant transformation, participate in intercellular adhesion and adhesion to extracellular matrix.
Synonyms: LAMP-1

Pathways: Autophagie