Syndecan 1 Antikörper

Produktnummer ABIN967377

Produktdetails anti-Syndecan 1 Antikörper

Target: Syndecan 1 (SDC1)

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Syndecan 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: BD Pharmingen™

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: NAMRU mouse mammary gland epithelial cell line NMuMG

Klon: 281

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: CD138 is expressed at low density on cells in the normal bone marrow, therefore, it may be desirable to amplify staining by using a biotinylated second-step antibody followed by a bright third-step reagent, such as Streptavidin-PE. Other reported applications include western blot analysis using a cationic blotting membrane and a specific method, ELISA, and immunohistochemical staining of acetone-fixed frozen and paraffin-embedded sections.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4° C and protected from prolonged exposure to light. Do not freeze.

Details zu Syndecan 1

Target: Syndecan 1 (SDC1)

Andere Bezeichnung: CD138 (SDC1 Produkte)

Synonyme: CG10497 antikoerper, CT27296 antikoerper, Dmel\\CG10497 antikoerper, Dsyn antikoerper, Syd antikoerper, Syn antikoerper, dSdc antikoerper, l(2)10608 antikoerper, sdc antikoerper, syd antikoerper, syn antikoerper, Syn1 antikoerper, cd138 antikoerper, synd1 antikoerper, xSyn1 antikoerper, syndecan1 antikoerper, Syndecan-1 antikoerper, XSyndecan-1 antikoerper, SDC1 antikoerper, CD138 antikoerper, SDC antikoerper, SYND1 antikoerper, syndecan antikoerper, AA408134 antikoerper, AA409076 antikoerper, Sstn antikoerper, Synd antikoerper, Synd1 antikoerper, syn-1 antikoerper, HSPG antikoerper, SYNDECA antikoerper, Syndecan antikoerper, Syndecan antikoerper, syndecan 1 antikoerper, Sdc antikoerper, sdc1 antikoerper, SDC1 antikoerper, Sdc1 antikoerper

Hintergrund: The 281-2 antibody reacts with the core protein of CD138 (Syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on B lymphocytes at specific stages during their differentiation: precursor B cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral B cells. It is thus implicated in mediating B cell-matrix interactions. CD138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mAb detects the Syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase. This antibody is routinely tested by flow cytometric analysis.
Synonyms: Syndecan-1

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism