PUMA Antikörper (C-Term)

Produktnummer ABIN966924

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-PUMA Antikörper (ABIN966924) detektiert spezifisch PUMA in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für PUMA Antikörper (C-Term) (ABIN966924)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PUMA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-PUMA Antikörper

Bindungsspezifität: C-Term

Kreuzreaktivität: Maus, Ratte (Rattus)

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Bcl-2-binding component 3 (BBC3) detection. Tested with WB in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human PUMA, identical to the related rat and mouse sequences.< br/>Immunogen was affinity purified.

Isotyp: IgG

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

Referenzen für anti-PUMA Antikörper (ABIN966924)

Chipuk, Bouchier-Hayes, Kuwana, Newmeyer, Green: "PUMA couples the nuclear and cytoplasmic proapoptotic function of p53." in: Science (New York, N.Y.), Vol. 309, Issue 5741, pp. 1732-5, (2005) (PubMed).

Details zu PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Andere Bezeichnung: Bcl-2-binding component 3 (BBC3)

Hintergrund: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91 % amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.

Pathways: p53 Signalweg, Positive Regulation of Endopeptidase Activity