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NUMA1 Antikörper (Cytoplasmic, Nuclear)

NUMA1 Reaktivität: Human ELISA, IHC (p), IHC (fro) Wirt: Maus Monoclonal 3-3A unconjugated
Produktnummer ABIN966706
  • Target Alle NUMA1 Antikörper anzeigen
    NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))
    Bindungsspezifität
    • 15
    • 15
    • 7
    • 7
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic, Nuclear
    Reaktivität
    • 82
    • 21
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 68
    • 14
    Maus
    Klonalität
    • 69
    • 13
    Monoklonal
    Konjugat
    • 36
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser NUMA1 Antikörper ist unkonjugiert
    Applikation
    • 30
    • 28
    • 26
    • 18
    • 17
    • 17
    • 13
    • 10
    • 8
    • 6
    • 2
    • 1
    • 1
    • 1
    ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Spezifität
    Reacts with an intracellular 228 kDa protein found inthe nucleus during interphase.
    Aufreinigung
    Concentrated.
    Immunogen
    Live Ls 174T cells (colon carcinoma).
    Klon
    3-3A
    Isotyp
    IgM
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  • Applikationshinweise
    Immunohistochemistry: 1:50-1:100 on frozen and formalin-fixed paraffin-embedded tissue sections. (For paraffin sections high temp. antigen demasker, 10mM citrate, pH 6.0, is required, (boiling tissue for 10 mins, followed by cooling for 10-20 mins).
    Western Blot 1:100-1:300.
    Staining Procedure: This antibody can be used on frozen and formalin tissue sections. Formalin-fixed paraffin-embdded tissue sections require, boilng the tisssue in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling to Rt for 10-20 mins. This step is done prior to antibody. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
    Kommentare

    Recommended Positive Control: Tonsil, Thymus, Spleen

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.2 mg/ml.
    Buffer
    20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C
  • Gueth-Hallonet, Weber, Osborn: "Cleavage of the nuclear matrix protein NuMA during apoptosis." in: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, (1997) (PubMed).

  • Target
    NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))
    Andere Bezeichnung
    NuMA (NUMA1 Produkte)
    Molekulargewicht
    228 kDa
    Gen-ID
    4926
    Pathways
    Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, M Phase
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