NEUROFILAMENTS Antikörper

Produktnummer ABIN966644

Produktdetails

Target: NEUROFILAMENTS

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), Western Blotting (WB)

Aufreinigung: Concentrated.

Immunogen: Neurofilament from Human Brain

Klon: 2F11

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100
WB: 1:100-1:200.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 in IHC . The optimal conditions should be determined by the individual laboratory.

Kommentare:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Human brain, IMR-5 cells

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/ml.

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen

Baldinger: "Sodium hyaluronate used to correct lens nucleus tumble." in: Ophthalmic surgery, Vol. 16, Issue 9, pp. 590, (1985) (PubMed).

Antigendetails

Target: NEUROFILAMENTS

Hintergrund: This antibody reacts with the 70 and 200 kD polypeptides of neurofilament. This antibody recognizes neurons, neuronal processes, and peripheral nerves, as well as, sympathetic ganglion cells and adrenal medulla. Cell body of neurons containing the non-phosphorylated neurofilamentis weakly stained.

Molekulargewicht: 68 kDa, 200 kDa(phosphorylated form)