COX1 Antikörper (N-Term)

Produktnummer ABIN965953

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch COX1 in WB. Er zeigt eine Reaktivität gegenüber Human und wurde in 4+ Publikationen erwähnt.

Kurzübersicht für COX1 Antikörper (N-Term) (ABIN965953)

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-COX1 Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Cytochrome c oxidase subunit 1 (MT-CO1) detection. Tested with WB in Human.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human CO1.< br/>Immunogen was affinity purified.

Isotyp: IgG

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

Referenzen für anti-COX1 Antikörper (ABIN965953)

Acín-Pérez, Bayona-Bafaluy, Bueno, Machicado, Fernández-Silva, Pérez-Martos, Montoya, López-Pérez, Sancho, Enríquez: "An intragenic suppressor in the cytochrome c oxidase I gene of mouse mitochondrial DNA." in: Human molecular genetics, Vol. 12, Issue 3, pp. 329-39, (2003) (PubMed).

Yamada, Andrews, Chan, McKeown, Magli, de Berardinis, Loewenstein, Lazar, OKeefe, Letson, London, Ruttum, Matsumoto, Saito, Morris, Del Monte, Johnson, Uyama, Houtman, de Vries, Carlow, Hart, Krawiecki, Shoffner, Vogel, Katowitz, Goldstein, Levin, Sener, : "Heterozygous mutations of the kinesin KIF21A in congenital fibrosis of the extraocular muscles type 1 (CFEOM1)." in: Nature genetics, Vol. 35, Issue 4, pp. 318-21, (2003) (PubMed).

Hosler, Ferguson-Miller, Calhoun, Thomas, Hill, Lemieux, Ma, Georgiou, Fetter, Shapleigh: "Insight into the active-site structure and function of cytochrome oxidase by analysis of site-directed mutants of bacterial cytochrome aa3 and cytochrome bo." in: Journal of bioenergetics and biomembranes, Vol. 25, Issue 2, pp. 121-36, (1993) (PubMed).

Kadenbach, Jarausch, Hartmann, Merle: "Separation of mammalian cytochrome c oxidase into 13 polypeptides by a sodium dodecyl sulfate-gel electrophoretic procedure." in: Analytical biochemistry, Vol. 129, Issue 2, pp. 517-21, (1983) (PubMed).

Details zu COX1

Target: COX1 (Cytochrome C Oxidase Subunit I (COX1))

Andere Bezeichnung: Cytochrome c oxidase subunit 1

Hintergrund: Cytochrome c oxidase subunit I (CO1 or MTCO1) is 1 of 3 mitochondrial DNA (mtDNA) encoded subunits (MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III (MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al. (2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase (COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant, cultured cells can maintain several mtDNA haplotypes at stable frequencies, the respiratory chain has little spare COX capacity, and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

Pathways: Proton Transport