CAPN1 Antikörper (AA 312-326)

Produktnummer ABIN965738

Der Polyklonal Anti-CAPN1-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, CAPN1 in Proben von Human, Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für CAPN1 Antikörper (AA 312-326) (ABIN965738)

Target: CAPN1 (Calpain 1 (CAPN1))

Reaktivität: Human, Maus, Ratte

Wirt: Bitte anfragen

Klonalität: Polyklonal

Konjugat: Dieser CAPN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CAPN1 Antikörper

Bindungsspezifität: AA 312-326

Immunogen: A synthetic peptide corresponding to a sequence at the middle region of human Calpain-1 (312-326 aa), different from the mouse sequence by two amino acids.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: 0.2ml of distilled water will yield a concentration of 500µg/ml.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Lagerung: -20 °C

Referenzen für anti-CAPN1 Antikörper (ABIN965738)

Imajoh, Aoki, Ohno, Emori, Kawasaki, Sugihara, Suzuki: "Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease." in: Biochemistry, Vol. 27, Issue 21, pp. 8122-8, (1989) (PubMed).

Details zu CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Andere Bezeichnung: Calpain 1

Hintergrund: Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1 (CAPN1) and L2. By quantitative RT-PCR, Ueyama et al. (1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis (ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al. (1989, 1990) assign CANPL1 to chromosome 11.