Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed

Produktnummer ABIN964951

Hersteller Produkt- Nr.: 610-1319-0100

Kurzübersicht für Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (HRP) - Preadsorbed (ABIN964951)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 610-1319-0100

Hersteller: Rockland

Verwendungszweck: Mouse IgG (H&L) Secondary Antibody Peroxidase Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG and Mouse Serum.  No reaction was observed against Human Serum Proteins.

Produktmerkmale: Anti-Mouse IgM (mu chain) peroxidase conjugated antibody generated in goat detects specifically mouse IgM.

Aufreinigung: HRP Mouse Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Mouse secondary antibody conjugated to peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for Immunohistochemistry, Western blotting as well as other antibody based assays. Immunohistochemistry Dilution: 1:1,000 - 1:8,000 Western Blot Dilution: 1:4,000 - 1:20,000 ELISA Dilution: 1:20,000 - 1:80,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Metzner, Luckert, Lemuth, Hämmerle, Moos: "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." in: Sensors (Basel, Switzerland), Vol. 18, Issue 5, (2018) (PubMed).

ONeill, Donohue, Omelchenko, Firestein: "The 3' UTRs of Brain-Derived Neurotrophic Factor Transcripts Differentially Regulate the Dendritic Arbor." in: Frontiers in cellular neuroscience, Vol. 12, pp. 60, (2018) (PubMed).

Shrestha, Ahn, Staples, Sathyan, Karpova, Foltz, Basrai: "Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells." in: Oncotarget, Vol. 8, Issue 29, pp. 46781-46800, (2018) (PubMed).

Castaño, Caviedes, Hirsch, Llanos, Iñiguez, Ronco: "Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels." in: PloS one, Vol. 12, Issue 1, pp. e0170389, (2017) (PubMed).

DLima, Ma, Winkler, Chu, Loh, Corpuz, Budnik, Lykke-Andersen, Saghatelian, Slavoff: "A human microprotein that interacts with the mRNA decapping complex." in: Nature chemical biology, Vol. 13, Issue 2, pp. 174-180, (2016) (PubMed).

Ray, Chopra, Long, Lahiri: "Human primary mixed brain cultures: preparation, differentiation, characterization and application to neuroscience research." in: Molecular brain, Vol. 7, pp. 63, (2015) (PubMed).

Hashimoto, Pais, Zhang, Saleh, Fu, Dai, Corrêa, Zheng, Cheng: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." in: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).

Frasca, Romero, Diaz, Alter-Wolf, Ratliff, Landin, Riley, Blomberg: "A molecular mechanism for TNF-α-mediated downregulation of B cell responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 1, pp. 279-86, (2012) (PubMed).

Frasca, Romero, Landin, Diaz, Riley, Blomberg: "Protein phosphatase 2A (PP2A) is increased in old murine B cells and mediates p38 MAPK/tristetraprolin dephosphorylation and E47 mRNA instability." in: Mechanisms of ageing and development, Vol. 131, Issue 5, pp. 306-14, (2010) (PubMed).

Ronsyn, Daans, Spaepen, Chatterjee, Vermeulen, DHaese, Van Tendeloo, Van Marck, Ysebaert, Berneman, Jorens, Ponsaerts: "Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord." in: BMC biotechnology, Vol. 7, pp. 90, (2008) (PubMed).

Williams, Weichert, Zakrzewicz, Da Silva-Azevedo, Pries, Baum, Egginton: "Differential gene and protein expression in abluminal sprouting and intraluminal splitting forms of angiogenesis." in: Clinical science (London, England : 1979), Vol. 110, Issue 5, pp. 587-95, (2006) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.