GDF15 Antikörper (C-Term)

Produktnummer ABIN964690

Hersteller Produkt- Nr.: 200-301-b07

Der Maus Monoklonal Anti-GDF15-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, GDF15 in Proben von Human zu detektieren.

Kurzübersicht für GDF15 Antikörper (C-Term) (ABIN964690)

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GDF15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 23B3-D2-H5

Produktdetails anti-GDF15 Antikörper

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 200-301-b07

Hersteller: Rockland

Verwendungszweck: GDF15 Antibody

Kreuzreaktivität (Details): This antibody reacts with the C-terminus of endogenous NAG-1 protein from human tissues.

Produktmerkmale: Synonyms: mouse anti-NAG1 Antibody, NAG-1, GDF15, MIC-1, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, prostate-derived factor

Aufreinigung: This product was purified from concentrated tissue culture supernatant Protein A chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This Protein A purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.

Immunogen Type: Recombinant Protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Application Note: This Protein A purified Anti-NAG1/GDF15 antibody has been tested by ELISA and western blotting for human NAG-1 protein.  For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG1/GDF15 (N-terminal), H variant or D variant specific antibodies.  Specific conditions for reactivity should be optimized by the end user.  Expect bands in Western blots of native protein of approximately 13 and 26 kDa in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:2,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.13 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu GDF15

Target: GDF15 (Growth Differentiation Factor 15 (GDF15))

Andere Bezeichnung: GDF15

Hintergrund: Background: Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

Gen-ID: 9518

UniProt: Q99988

Pathways: SARS-CoV-2 Protein Interaktom