Fluorescein Antikörper (Biotin)

Produktnummer ABIN964654

Hersteller Produkt- Nr.: 700-106-096

Dieser Ziege Polyklonal Antikörper detektiert spezifisch Fluorescein in WB, ELISA und IHC.

Kurzübersicht für Fluorescein Antikörper (Biotin) (ABIN964654)

Target: Fluorescein

Fragment: F(ab')2 fragment

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails

Hersteller Produkt- Nr.: 700-106-096

Hersteller: Rockland

Verwendungszweck: F(Ab')2 Fluorescein Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum and Fluorescein conjugated BSA.

Produktmerkmale: Synonyms: goat F(ab')2 anti-Fluorescein Antibody biotin Conjugation, goat F(ab')2 anti-Fluorescein biotin Conjugated Antibody, goat F(ab')2 anti-FITC Antibody biotin Conjugation

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen:

Immunogen: Fluorescein conjugated to Goat IgG

Immunogen Type: Other

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: F(ab')2 Anti-Fluorescein Antibody Conjugated to biotin has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: Fluorescein

Substanzklasse: Chemical

Hintergrund: Background: F(ab')2 Anti-Fluorescein Antibody generated in goat detects fluorescein. It is an organic dye used as a fluorescent tracer in many application including cellular staining. This F(ab')2 Anti-Fluorescein Antibody is Biotin conjugated.