Fluorescein Antikörper
Kurzübersicht für Fluorescein Antikörper (ABIN964651)
Target
Fragment
Wirt
Klonalität
Konjugat
Applikation
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Hersteller Produkt- Nr.
- 700-101-096
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Hersteller
- Rockland
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Verwendungszweck
- F(Ab')2 Fluorescein Antibody
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Kreuzreaktivität (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and Fluorescein conjugated BSA.
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Produktmerkmale
- Synonyms: goat F(ab')2 anti-Fluorescein Antibody, goat F(ab')2 anti-FITC Antibody
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Aufreinigung
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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Sterilität
- Sterile filtered
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Immunogen
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Immunogen: Fluorescein conjugated to Goat IgG
Immunogen Type: Other
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Isotyp
- IgG
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Applikationshinweise
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Immunohistochemistry Dilution: User Optimized
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot Dilution: 1:200 - 1:1,000
ELISA Dilution: 1:2,000 - 1:8,000
Other: User Optimized
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Haltbarkeit
- 12 months
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- Fluorescein
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Substanzklasse
- Chemical
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Hintergrund
- Background: F(ab')2 Anti-Fluorescein Antibody generated in goat detects fluorescein. It is an organic dye used as a fluorescent tracer in many application including cellular staining.
Target
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