ODF2 Antikörper

Produktnummer ABIN964607

Hersteller Produkt- Nr.: 200-401-c43

Der Kaninchen Polyklonal Anti-ODF2-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, ODF2 in Proben von Maus zu detektieren.

Kurzübersicht für ODF2 Antikörper (ABIN964607)

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ODF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-ODF2 Antikörper

Hersteller Produkt- Nr.: 200-401-c43

Hersteller: Rockland

Verwendungszweck: Cenexin-1 Antibody

Kreuzreaktivität (Details): It is directed against, and shows specific reactivity for, human Cenexin-1 protein.

Produktmerkmale: Synonyms: rabbit anti-Cenexin-1 Antibody, ODF84 antibody, Outer dense fiber of sperm tail 2 antibody, Cenexin1 variant 1 antibody, KKT 4 antibody, Cenexin 1

Aufreinigung: This antibody was purified from monospecific antiserum by protein-A purified immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a truncated recombinant protein hCenexin1.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Cenexin-1 purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 93 kDa in size corresponding to Cenexin-1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500-1:2000

ELISA Dilution: 1:500 - 1:10,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.53 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu ODF2

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Andere Bezeichnung: ODF2

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Cenexin-1 (ODF84) and cenexin-2 are splicing variants that are expressed from the same genetic locus Outer Dense Fiber protein 2 (ODF2) . Cenexin-1 and cenexin-2, regarded as centriolar proteins. Cenexin-1, a 93-kD variant of ODF2, is the major form of cennexin expressed in various somatic cells and tissues and associates with centrosomes. Cenexin-1 contains a unique C-terminal extension that appears to play a critical role in recruiting mitotic regulators such as polo-like kinase 1. In contrast, ODF2, a 70- kDa protein, is a major component of sperm tail cytoskeleton and abundantly expressed in testis. Additional evidence suggests that both of these proteins play important role in primary cilia formation. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation.  Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. Cenexin-1 is one of the major outer dense fiber proteins. Multiple protein isoforms are encoded by transcript variants of the cenexin gene, however, not all isoforms and variants have been fully described.

Gen-ID: 4957

NCBI Accession: NP_702915

UniProt: Q5BJF6

Pathways: M Phase