CCL2 Antikörper

Produktnummer ABIN964602

Hersteller Produkt- Nr.: 200-401-b60

Dieses Anti-CCL2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CCL2 in WB und ELISA. Geeignet für Rind (Kuh).

Kurzübersicht für CCL2 Antikörper (ABIN964602)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-CCL2 Antikörper

Hersteller Produkt- Nr.: 200-401-b60

Hersteller: Rockland

Verwendungszweck: CCL2 Antibody

Kreuzreaktivität (Details): This antibody is specific for bovine CCL2 protein.

Produktmerkmale: Synonyms: rabbit anti-CCL2 antibody, CCL-2, CCL 2, Chemokine (C-C motif) ligand 2, monocyte chemotactic (or chemoattractant) protein-1 (MCP-1)

Aufreinigung: This product was Protein-A purified from monospecific antiserum by chromatography.

Immunogen:

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to the 76 amino acids of the mature bovine CCL2 protein.

Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This protein-A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.8 kDa in size corresponding to bovine CCL2 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:4,500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Andere Bezeichnung: CCL2

Hintergrund: Background: CCL2 is a small cytokine belonging to the CC chemokine family that recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. This chemokine is produced as a protein precursor containing a signal peptide of 23 amino acids and a mature peptide of 76 amino acids. It is a monomeric polypeptide, with a total molecular weight of approximately 8.8 kDa. The cell surface receptors that bind CCL2 are CCR2 and CCR4. CCL2 is found at the site of tooth eruption and bone degradation. In the bone, CCL2 is expressed by mature osteoclasts and osteoblasts and is under the control of nuclear factor kB (NFkB). In human osteoclasts, CCL2 and RANTES (regulated on activation normal T cell expressed and secreted) may act as an autocrine loop in human osteoclast differentiation. CCL2 causes the degranulation of basophils and mast cells, an effect potentiated by pre-treatment with IL-3 and other cytokines.

Gen-ID: 281043, 27806711

UniProt: A5PJF7

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylierungen bei SARS-CoV-2 Infektion