Vitamin D Receptor Antikörper (AA 89-105)

Produktnummer ABIN962160

Der Ratte Monoklonal anti-Vitamin D Receptor Antikörper (ABIN962160) detektiert spezifisch Vitamin D Receptor in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Vitamin D Receptor Antikörper (AA 89-105) (ABIN962160)

Target: Vitamin D Receptor (VDR)

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser Vitamin D Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Vitamin D Receptor Antikörper

Bindungsspezifität: AA 89-105

Spezifität: Recognizes both the occupied and unoccupied forms of VDR. Does not cross-react with estrogen and glucocorticoid receptors. In mammals, recognizes a protein of ~53kD which is identified as vitamin D Receptor (VDR). In avians, VDR appears as a doublet of 58 and 60kD. Species cross-reactivity: Human, porcine, mouse, rat, hamster, frog, chicken and fish.

Aufreinigung: Protein G purified

Immunogen: Vitamin D receptor protein from chicken intestine (53kD). Cellular Localization: Nuclear. Epitope: 89-105.

Type of Immunogen: Purified protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Positive control: LNCaP and HeLa cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide. Sourced in Ascites.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Details zu Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Andere Bezeichnung: Vitamin D Receptor / VDR

Substanzklasse: Chemical

Hintergrund: Name/Gene ID: VDR
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: VDR, NR1I1, Vitamin D3 receptor, Vitamin D receptor

Gen-ID: 7421

UniProt: P11473

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway