alpha Tubulin Antikörper (C-Term, Subunit alpha)

Produktnummer ABIN962076

Dieses Anti-alpha Tubulin-Antikörper ist ein Ratte Monoklonal-Antikörper zur Detektion von alpha Tubulin in WB, ELISA, IHC, IHC (p), IP, IHC (fro) und RIA. Geeignet für Human, Saccharomyces cerevisiae, Säugetier, Avian und Echinodermata.

Kurzübersicht für alpha Tubulin Antikörper (C-Term, Subunit alpha) (ABIN962076)

Target: alpha Tubulin (TUBA1)

Reaktivität: Human, Saccharomyces cerevisiae, Säugetier, Avian, Echinodermata

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser alpha Tubulin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Klon: YL1-2

Produktdetails anti-alpha Tubulin Antikörper

Bindungsspezifität: C-Term, Subunit alpha

Marke: IHC-plus™

Spezifität: Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin.

Aufreinigung: Affinity purified

Immunogen: Yeast tubulin.

Type of Immunogen: Purified protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:100 - 1:1000), IHC, IHC-Fr, IHC-P (5 μg/mL), IP, RIA, WB

Kommentare:

Target Species of Antibody: Yeast

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu alpha Tubulin

Target: alpha Tubulin (TUBA1)

Andere Bezeichnung: Alpha Tubulin

Pathways: Microtubule Dynamics