KLF4 Antikörper (AA 80-129)

Produktnummer ABIN960992

Der Kaninchen Polyklonal Anti-KLF4-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, KLF4 in Proben von Human, Rind (Kuh), Pferd, Hund, Schaf, Schwein und Affe zu detektieren.

Kurzübersicht für KLF4 Antikörper (AA 80-129) (ABIN960992)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reaktivität: Human, Rind (Kuh), Pferd, Hund, Schaf, Schwein, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KLF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-KLF4 Antikörper

Bindungsspezifität: AA 80-129

Marke: IHC-plus™

Spezifität: Human KLF4

Homologie: Percent identity by BLAST analysis: Human (100%) Dog, Horse (86%) Sheep, Bovine, Pig (80%).

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide located between aa80-129 of human KLF4 (O43474, NP_004226). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Marmoset (100%), Galago, Dog, Bat, Horse (86%), Sheep, Elephant, Panda, Cat, Bovine, Pig (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (2.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Andere Bezeichnung: KLF4

Hintergrund: Name/Gene ID: KLF4

Synonyms: KLF4, EZF, GKLF, Kruppel-like factor 4 (gut), Hezf, Krueppel-like factor 4

Gen-ID: 9314

NCBI Accession: NP_004226

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance