Caspase 2 Antikörper (N-Term)

Produktnummer ABIN96091

Hersteller Produkt- Nr.: 600-401-412

Produktdetails anti-Caspase 2 Antikörper

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Bindungsspezifität: N-Term

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Caspase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 600-401-412

Hersteller: Rockland

Verwendungszweck: Caspase-2 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against rat and mouse caspase-2. The antibody detects long and short forms of the protein.

Produktmerkmale: Synonyms: rabbit anti-Caspase-2 Antibody, Apoptosis related cysteine peptidase antibody, CASP-2, CASP 2 antibody, Casp 2L Pro antibody, CASP2 antibody, Caspase 2 apoptosis related cysteine protease antibody, Caspase2 antibody, Caspase-2, Developmentally down regulated 2 antibody, ICH 1 antibody, ICH-1, Caspase-2 subunit p18, Caspase-2 subunit p13, Caspase-2 subunit p12

Aufreinigung: Affinity purified antibody

Sterilität: Sterile filtered

Immunogen:

Immunogen: A synthetic peptide corresponding to the N-terminus of rat Caspase-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH) through a cysteine residue linker.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: This affinity purified polyclonal antibody was tested by ELISA and immunoblotting and was found to be reactive with 48 kDa mouse and rat caspase-2.  A control and rat caspase-2 transfected human 293 cell lysate was used to show by immunoblotting that this antibody detects rat caspase-2.  No reaction was observed against human caspase-2.  Immunoblot of mouse neuronal cell lysates from wild-type and caspase-2 (-/-) mice (not shown) was used to show that this antibody cross reacts with mouse caspase-2. A 1:750 dilution is recommended for immunoblot. This product was assayed against 1.0 μg of immunizing peptide in a standard sandwich ELISA using HRP conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and TMB as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,500 to 1:6,000 is suggested for this product. 

Western Blot Dilution: 1:750

ELISA Dilution: 1:1,500 - 1:6,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.44 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Caspase 2

Target: Caspase 2 (CASP2) (Caspase 2, Apoptosis-Related Cysteine Peptidase (CASP2))

Andere Bezeichnung: Casp2 (CASP2 Produkte)

Hintergrund: Background: Apoptosis occurs during normal cellular development and involves dramatic changes in cellular structure.  Disruption of apoptosis may contribute to cancer and other diseases.  Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. Caspase-2 has been called ICH-1 in human, CED-3 in C.elegans and NEDD-2 in mouse.  Caspase-2 is synthesized as an inactive precursor that is processed in cells undergoing apoptosis.  The precursor form of all caspases is composed of a pro-domain, and large and small catalytic subunits.  The active forms of caspases are generated by several stimuli, including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions.  Caspase-2 is activated in vitro by caspase-1, caspase-3 and granzyme B.  Caspase-2 functions as an upstream apoptosis initiator that participates in the activation of downstream caspases, such as caspase-3, caspase-6 and caspase-7.  Caspase-2 is highly expressed in embryonic mouse brain but not in adult neural tissue.  Alternative mRNA splicing produces a long (48 kDa) and short (35 kDa) form of caspase-2.  These forms show tissue specificity and function either as inducers or suppressors of apoptosis.  Mature caspase-2 is 435 amino acids in length cleaved (~50 kDa) and is cleaved upon activation into 10 and 20 kDa subunits.

Gen-ID: 64314

UniProt: P55215

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Neurotrophin Signalübertragung