ELANE Antikörper (N-Term)

Produktnummer ABIN953688

Dieses Anti-ELANE-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ELANE in WB und EIA. Geeignet für Human.

Kurzübersicht für ELANE Antikörper (N-Term) (ABIN953688)

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ELANE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-ELANE Antikörper

Bindungsspezifität: AA 20-47, N-Term

Spezifität: This antibody recognizes human Neutrophil elastase at N-term.

Aufreinigung: Purified through a Protein A column followed by peptide affinity purification

Immunogen: Synthetic peptide - KLH conjugated - corresponding to the N-terminal region (between 20-47aa ) of human Neutrophil elastase

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ELANE

Target: ELANE (Elastase, Neutrophil Expressed (ELANE))

Andere Bezeichnung: Neutrophil Elastase

Hintergrund: Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.Synonyms: Bone marrow serine protease, ELA2, ELANE, Elastase-2, HLE, Human leukocyte elastase, Medullasin, Neutrophil elastase, PMN elastase

Molekulargewicht: 28518 Da

Gen-ID: 1991

NCBI Accession: NP_001963