Der Huhn Polyklonal anti-ENO2/NSE Antikörper (ABIN953676) detektiert spezifisch ENO2/NSE in WB, IF und IHC (p).
Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.
Chickens were immunized with two synthetic peptide KLH conjugates corresponding to different regions of the NSE-2 gene product, but are shared between the Human (NP_001966, NCBI) and Rat (AAA41119) sequences. Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of each of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Human NSE-2 (EC 4.2.1.11) is a 47,138 dalton protein (434 amino acids) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE-2 catalyzes the conversion of 2-phospho-D-glycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues.Synonyms: 2-phospho-D-glycerate hydro-lyase, ENO2, Enolase 2, Gamma-enolase, NSE, Neural enolase