GLB1 Antikörper

Produktnummer ABIN950653

Produktdetails anti-GLB1 Antikörper

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Reaktivität: E. coli

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser GLB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody recognizes the LacZ gene product with high titre and high specificity. This anitbody can be used together with Rabbit and Mouse primaty antibodies for Double Immunostaining with no Secondary antibody cross reactivity. It even works well with fusion protein of LacZ! Quality Control: This antibody preparation was analyzed by Immunohistochemistry (at a dilution of 1/5000) using tissues infected with a virus bearing the LacZ gene. Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FC-N, 1/2000 dilution) was used as a secondary antibody.

Aufreinigung: Purified

Immunogen: Purified Beta-Galactosidase (LacZ) protein emulsified in Freund's adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using Beta-Galactosidase conjugated to an Agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 myg/ml) and purified IgY (10 mg/ml), and is filter-sterilized.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10.0 mg/mL (based on absorbance at 280 nm)

Buffer: Sodium Phosphate-Buffered (10 mM) isotonic (0.9 % , w/v) Saline (PBS, pH 7.2) (50 % by volume) with 50 % Glycerol as an anti-freezing agent and 0.01 % Thimerosal as preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial before opening.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody at -20 °C in the dark.

Details zu GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Andere Bezeichnung: beta Galactosidase (GAL) (GLB1 Produkte)

Hintergrund: Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.Synonyms: Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag

Gen-ID: 945006

NCBI Accession: AP_000996

Pathways: Glycosaminoglycan Metabolic Process