His Tag Antikörper

Produktnummer ABIN950190

Dieses Anti-His Tag-Antikörper ist ein Huhn Polyklonal-Antikörper zur Detektion von His Tag in WB. Geeignet für .

Kurzübersicht für His Tag Antikörper (ABIN950190)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser His Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-His Tag Antikörper

Sequenz: HHHHHH

Aufreinigung: Purified

Immunogen: 6X-Histidine peptide conjugated to Keyhole Limpet Hemocyanin protein and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with Glycerol and Thimerosal, and filter-sterilized.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL (based on absorbance at 280 nm)

Buffer: Sodium Phosphate-Buffered (10 mM) isotonic (0.9 % , w/v) Saline (PBS, pH 7.2) (50 % by volume) with 50 % Glycerol as an anti-freezing agent and 0.01 % Thimerosal as preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu His Tag

Target: His Tag

Andere Bezeichnung: 6xHistidine Epitope Tag

Substanzklasse: Tag

Hintergrund: In the last couple of years many peptide sequences/epitopes for the purification of recombinant proteins have been established. These so-called Synonyms: 6xHis-Tag, HHHHHH Tag, HIS6 Tag, His Tag