Amylase, alpha Antikörper

Produktnummer ABIN95004

Hersteller Produkt- Nr.: 200-401-045

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Amylase, alpha in WB, ELISA und IP. Er zeigt eine Reaktivität gegenüber Bacillus amyloliquefaciens.

Kurzübersicht für Amylase, alpha Antikörper (ABIN95004)

Target: Amylase, alpha (AMY)

Reaktivität: Bacillus amyloliquefaciens

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Amylase, alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Produktdetails anti-Amylase, alpha Antikörper

Hersteller Produkt- Nr.: 200-401-045

Hersteller: Rockland

Verwendungszweck: Alpha Amylase Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens].

Produktmerkmale: Synonyms: rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase

Aufreinigung: Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen:

Immunogen: a-Amylase [Bacillus amyloliquefaciens]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Alpha Amylase has been tested by ELISA and western blot and is assayed against 1.0 μg of a-Amylase [Bacillus amyloliquefaciens] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Alpha Amylase Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Alpha Amylase Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Amylase, alpha

Target: Amylase, alpha (AMY)

Andere Bezeichnung: Alpha-amylase

Hintergrund: Background: Alpha Amylase is a protein enzyme that preforms endohydrolysis of alpha bonds in large alpha linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Alpha Amylase applications include ethanol production and reduction of starch or glycogen molecules. Anti-Alpha Amylase Antibody is useful for researchers interested in Metabolism, Cancer, Cell Signaling, and Signal Transduction research.

Gen-ID: 7849308

NCBI Accession: WP_013352208

UniProt: P00692