Amylase, alpha Antikörper (Biotin)

Produktnummer ABIN1607777

Produktdetails anti-Amylase, alpha Antikörper (Biotin)

Target: Amylase, alpha (AMY)

Reaktivität: Bacillus amyloliquefaciens

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Amylase, alpha Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against a-Amylase from other sources may occur but has not been specifically determined.

Reinheit: Anti-Alpha Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.amyloliquefaciens].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: a-Amylase [Bacillus amyloliquefaciens]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Alpha Amylase has been assayed against 1.0 µg of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Amylase, alpha

Target: Amylase, alpha (AMY)

Andere Bezeichnung: Alpha Amylase (AMY Produkte)

Synonyme: LOC663954 antikoerper, LOC100136478 antikoerper, alpha amylase antikoerper, alpha-amylase antikoerper, LOC663954 antikoerper, HQ_RS05305 antikoerper, LOC100136478 antikoerper

Hintergrund: Alpha Amylase is a member of the glycosyl hydrolase 13 family, involved primarily in the hydrolysis of biopolymer residues of 1, 4-alpha-D-glucosidic linkages in polysaccharides. It performs its catalytic function in metabolism of carbohydrates. Anti-Alpha Amylase antibody is ideal for investigators involved in Metabolism, Signal Transduction, and Cancer research. Synonyms: Alpha-amylase EC=3.2.1.1 1,4-alpha-D-glucan glucanohydrolase

Gen-ID: 7849308

UniProt: P00692