SIT1 Antikörper

Produktnummer ABIN94465

Dieser Maus Monoklonal Antikörper erkennt spezifisch SIT1 in WB, IP und ICFC. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für SIT1 Antikörper (ABIN94465)

Target: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SIT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Intracellular Flow Cytometry (ICFC)

Klon: SIT-01

Produktdetails anti-SIT1 Antikörper

Verwendungszweck: Anti-SIT Purified

Spezifität: The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.

Kreuzreaktivität (Details): Human

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Bacterially produced recombinant intracellular fragment of human SIT.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-5 μg/mL, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-SIT1 Antikörper (ABIN94465)

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, (2004) (PubMed).

Details zu SIT1

Target: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Andere Bezeichnung: SIT

Hintergrund: Signaling threshold regulating transmembrane adapt,SIT is a 21 kDa transmembrane adaptor protein expressed exclusively in lymphoid organs. It acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.,SIT1

Gen-ID: 27240

UniProt: Q9Y3P8