Acrosin Antikörper (ACR)

Details for Product anti-ACR Antibody No. ABIN94455, Anbieter: Anmelden zum Anzeigen
Antigen
  • ACR
  • MGC130972
  • Acro
  • Acrosin
  • acrosin
  • acrosin L homeolog
  • acrosin prepropeptide
  • ACR
  • acr.L
  • Acr
Reaktivität
Schwein
51
24
19
19
2
2
2
2
Wirt
Maus
53
21
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser Acrosin Antikörper ist unkonjugiert
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
57
23
19
19
18
15
13
11
5
4
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Klon ACR-2
Isotyp IgG1
Spezifität The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
Keine Kreuzreaktivität Human, Rind (Kuh), Hund
Reinigung Purified from ascites by protein-A affinity chromatography.
Reinheit > 95 % (by SDS-PAGE)
Plasmids, Primers & others Plasmide, Primers & weitere Acrosin products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung ACR (ACR Antibody Abstract)
Pathways cAMP Metabolic Process
Applikations-hinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Produkt verwendet in: Ded, Dostalova, Dorosh, Dvorakova-Hortova, Peknicova: "Effect of estrogens on boar sperm capacitation in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 8, pp. 87, 2010 (PubMed).

Peknicova, Capkova, Geussova, Ivanova, Mollova: "Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro." in: Theriogenology, Vol. 56, Issue 2, pp. 211-23, 2001 (PubMed).

Moos, Peknicova, Tesarik: "Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction." in: Biology of reproduction, Vol. 49, Issue 2, pp. 408-15, 1993 (PubMed).

Moos, Peknicova, Tesarik: "Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction." in: Biochimica et biophysica acta, Vol. 1176, Issue 3, pp. 199-207, 1993 (PubMed).

Moos, Tesarik, Leca, Peknicova: "Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin." in: FEBS letters, Vol. 294, Issue 1-2, pp. 27-30, 1992 (PubMed).

Peknicová, Moos: "Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test." in: Andrologia, Vol. 22, Issue 5, pp. 427-35, 1991 (PubMed).

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