Acrosin Antikörper

Produktnummer ABIN94455

Dieses Anti-Acrosin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Acrosin in WB, FACS und ICC. Geeignet für Schwein. Dieses Primary Antibody wurde in 7+ Publikationen zitiert.

Kurzübersicht für Acrosin Antikörper (ABIN94455)

Target: Acrosin (ACR)

Reaktivität: Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Acrosin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: ACR-2

Produktdetails anti-Acrosin Antikörper

Verwendungszweck: Anti-Por Acrosin Purified

Spezifität: The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.

Keine Kreuzreaktivität: Rind (Kuh), Hund, Human

Kreuzreaktivität (Details): Porcine

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-12 μg/mL. Intracellular staining.
Immunocytochemistry: Membrane permeabilization (acetone) is essential.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-Acrosin Antikörper (ABIN94455)

Ded, Dostalova, Zatecka, Dorosh, Komrskova, Peknicova: "Fluorescent analysis of boar sperm capacitation process in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 17, Issue 1, pp. 109, (2020) (PubMed).

Ded, Dostalova, Dorosh, Dvorakova-Hortova, Peknicova: "Effect of estrogens on boar sperm capacitation in vitro." in: Reproductive biology and endocrinology : RB&E, Vol. 8, pp. 87, (2010) (PubMed).

Peknicova, Capkova, Geussova, Ivanova, Mollova: "Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro." in: Theriogenology, Vol. 56, Issue 2, pp. 211-23, (2001) (PubMed).

Moos, Peknicova, Tesarik: "Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction." in: Biology of reproduction, Vol. 49, Issue 2, pp. 408-15, (1993) (PubMed).

Moos, Peknicova, Tesarik: "Relationship between molecular conversions of acrosin and the progression of exocytosis in the calcium ionophore-induced acrosome reaction." in: Biochimica et biophysica acta, Vol. 1176, Issue 3, pp. 199-207, (1993) (PubMed).

Moos, Tesarik, Leca, Peknicova: "Mechanism of maturation and nature of carbohydrate chains of boar sperm acrosin." in: FEBS letters, Vol. 294, Issue 1-2, pp. 27-30, (1992) (PubMed).

Peknicová, Moos: "Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test." in: Andrologia, Vol. 22, Issue 5, pp. 427-35, (1991) (PubMed).

Details zu Acrosin

Target: Acrosin (ACR)

Andere Bezeichnung: Acrosin

Hintergrund: Acrosin,Acrosin is a serine proteinase expressed in the acrosome of mature spermatozoa. This enzyme facilitates penetration of the sperm through the zona pellucida of the ovum.,ACR, FBP53

Gen-ID: 397098

UniProt: P08001

Pathways: cAMP Metabolic Process