GAPDHS Antikörper

Produktnummer ABIN94395

Dieses Anti-GAPDHS-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von GAPDHS in WB, FACS und ICC. Geeignet für Human und Schwein.

Kurzübersicht für GAPDHS Antikörper (ABIN94395)

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Reaktivität: Human, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GAPDHS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: Hs-8

Produktdetails anti-GAPDHS Antikörper

Verwendungszweck: Anti-GAPDHS Purified

Spezifität: The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.

Kreuzreaktivität (Details): Human, Porcine

Aufreinigung: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: Recommended dilution: 10 μg/mL, membrane permeabilization (acetone) is essential.
Flow cytometry: Recommended dilution: 3-12 μg/mL. Intraacrosomal staining.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu GAPDHS

Target: GAPDHS (Glyceraldehyde-3-Phosphate Dehydrogenase, Spermatogenic (GAPDHS))

Andere Bezeichnung: GAPDHS

Hintergrund: Glyceraldehyde-3-phosphate dehydrogenase, spermato,GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,SGAPDH, HSD-35

Gen-ID: 26330

UniProt: O14556

Pathways: Regulation of Carbohydrate Metabolic Process