PAG1 Antikörper (C-Term)

Produktnummer ABIN94270

Produktdetails anti-PAG1 Antikörper

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: The antibody PAG-C1 recognizes an epitope located in the intracellular C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Kreuzreaktivität (Details): Mouse, Human, Rat, Bovine, Other not tested

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.

Klon: PAG-C1

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (paraffin sections): Positive tissue: appendix (germinal center of lymphatic follicle), heat-mediated antigen retrieval in citrate buffer pH 6.1.
Immunoprecipitation: Positive control: RAJI human Burkitt lymphoma cell line.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-PAG1 Antikörper (ABIN94270)

Davidson, Bakinowski, Thomas, Horejsi, Veillette: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, (2003) (PubMed).

Vang, Abrahamsen, Myklebust, Horejsí, Taskén: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, (2003) (PubMed).

Details zu PAG1

Target: PAG1 (phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1))

Andere Bezeichnung: PAG / Cbp (PAG1 Produkte)

Hintergrund: Phosphoprotein membrane anchor with glycosphingoli,PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.,CBP, PAG

Gen-ID: 94212

UniProt: Q3U1F9

Pathways: p53 Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, CXCR4-mediated Signaling Events