Transferrin Receptor Antikörper (PE)

Produktnummer ABIN94224

Zitiert in 1 Publikation. Der PE-konjugierte Maus Monoklonal anti-Transferrin Receptor Antikörper (Klon MEM-75) (ABIN94224) detektiert spezifisch Transferrin Receptor in FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Transferrin Receptor Antikörper (PE) (ABIN94224)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Receptor Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Klon: MEM-75

Produktdetails anti-Transferrin Receptor Antikörper (PE)

Verwendungszweck: Anti-Hu CD71 PE

Kreuzreaktivität (Details): Human

Produktmerkmale: The antibody MEM-75 reacts with an extracellular epitope of CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages and erythroid precursors, it is lost on resting blood leukocytes. The antibody MEM-75 does not block binding of transferrin to the receptor.

Aufreinigung: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: NALM-6 human pre-B cell line

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze,Protect from prolonged exposure to light

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen für anti-Transferrin Receptor Antikörper (PE) (ABIN94224)

Beck, Balogh, Kis, Izsépi, Cervenak, László, Bíró, Liliom, Mocsár, Vámosi, Füst, Matko: "New cholesterol-specific antibodies remodel HIV-1 target cells' surface and inhibit their in vitro virus production." in: Journal of lipid research, Vol. 51, Issue 2, pp. 286-96, (2010) (PubMed).

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: CD71

Hintergrund: Transferrin receptor,CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH . CD71 is also involved in uptake of non-transferrin bound iron.,TfR1, TfR, TR, Trfr, T9, p90, TFRC

Gen-ID: 7037

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis