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CD59 Antikörper

Dieses Anti-CD59-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD59 in WB, FACS, IP und IHC (p). Geeignet für Human und Maus. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.
Produktnummer ABIN94203

Kurzübersicht für CD59 Antikörper (ABIN94203)

Target

Alle CD59 Antikörper anzeigen
CD59

Reaktivität

  • 159
  • 16
  • 13
  • 11
  • 5
  • 5
  • 5
  • 1
  • 1
Human, Maus

Wirt

  • 97
  • 93
  • 8
  • 6
Maus

Klonalität

  • 106
  • 95
  • 1
Monoklonal

Konjugat

  • 105
  • 25
  • 14
  • 13
  • 8
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser CD59 Antikörper ist unkonjugiert

Applikation

  • 106
  • 95
  • 75
  • 47
  • 46
  • 41
  • 33
  • 23
  • 16
  • 14
  • 13
  • 13
  • 11
  • 11
  • 8
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon

MEM-43-5
  • Verwendungszweck

    Anti-Hu CD59 Purified

    Spezifität

    The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored extracellular glycoprotein expressed on the surface of all hematopoietic cells, it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies.

    Kreuzreaktivität (Details)

    Mouse, Human

    Aufreinigung

    Purified by protein-A affinity chromatography.

    Reinheit

    > 95 % (by SDS-PAGE)

    Immunogen

    Thymocytes and T lymphocytes

    Isotyp

    IgG2b
  • Applikationshinweise

    Flow cytometry: Recommended dilution: 0.5-4 μg/mL, positive control: blood.
    Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/mL.
    Western blotting: Recommended dilution: 1-2 μg/mL, positive control: mouse spleen, non-reducing conditions.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Do not freeze.

    Lagerung

    4 °C

    Informationen zur Lagerung

    Store at 2-8°C. Do not freeze.
  • Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, (2007) (PubMed).

    Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, (1997) (PubMed).

    Bodian, Davis, Morgan, Rushmere: "Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59." in: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 507-16, (1997) (PubMed).

  • Target

    CD59

    Andere Bezeichnung

    CD59

    Hintergrund

    CD59 Molecule,CD59 (protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.,MACIF, MAC-IP, protectin, MIRL, HRF-20, HRF20, 1F5, MIN

    Gen-ID

    966

    UniProt

    P13987

    Pathways

    Komplementsystem
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