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beta-2 Microglobulin Antikörper (Biotin)

Name: beta-2 Microglobulin Antikörper (Biotin)
Brand: antibodies-online
SKU: ABIN93901
Price: 463.75 EUR
Availability: InStock

B2M Reaktivität: Human, Schwein WB, FACS, ELISA, IHC (p) Wirt: Maus Monoclonal B2M-02 Biotin

Flow cytometry (surface staining) of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02 biotin. (beta-2 Microglobulin Antikörper (Biotin))

FACS

Surface staining of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02 . (beta-2 Microglobulin Antikörper (Biotin))

FACS

Surface staining of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02 biotin. (beta-2 Microglobulin Antikörper (Biotin))

FACS

3 Abbildungen

(2 Referenzen)

Produktnummer ABIN93901

Datenblatt (PDF)

Target Alle beta-2 Microglobulin (B2M) Antikörper anzeigen

beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
Reaktivität
214

52

30

27

9

4

3

3

3

2

2

1

1

1

1
Human, Schwein
Wirt
126

117

9

6

2

1
Maus
Passende Sekundärantikörper
Klonalität
134

126

1
Monoklonal
Konjugat
117

31

26

14

14

8

3

3

3

3

3

3

3

3

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1
Dieser beta-2 Microglobulin Antikörper ist konjugiert mit Biotin
Applikation
130

108

77

67

60

40

39

30

26

18

17

14

13

10

6

4

4

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck

Anti-Hu beta2-Microglobulin Biotin

Spezifität

The mouse monoclonal antibody B2M-02 reacts with beta2-microglobulin (beta2M), an extracellular antigen associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

Kreuzreaktivität (Details)

Human, Porcine

Aufreinigung

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen

Purified isolated human beta2-microglobulin.

Klon

B2M-02

Isotyp

IgG1

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anti-beta-2-Microglobulin (B2M) antibody (Biotin)
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Applikationshinweise

Flow cytometry: Recommended dilution: 1-2 μg/mL, positive control: PBL cell line, negative control: DAUDI human lymphoma cell line, erythrocytes.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Do not freeze.
Avoid prolonged exposure to light.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
Drbal, Angelisová, Hilgert, Cerný, Novák, Horejsí: "A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells." in: Blood, Vol. 98, Issue 5, pp. 1561-6, (2001) (PubMed).

Hilgert, Horejsí, Kristofová: "The use of murine monoclonal antibody B2M-01 for detection and purification of human beta 2-microglobulin." in: Folia biologica, Vol. 30, Issue 6, pp. 369-76, (1985) (PubMed).
Target

beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Andere Bezeichnung

beta2-Microglobulin (B2M Produkte)

Hintergrund

Beta-2-microglobulin,Beta2-microglobulin non-covalently associates with the 44 kDa alpha chain to forms the HLA Class I antigen complex. Human beta2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.,B2M

Gen-ID

567

UniProt

P61769

Pathways

T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process