WB: 1:1000-3000 Optimal conditions should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handhabung
Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
Target
DYKDDDDK Tag
Andere Bezeichnung
DYKDDDD-Tag
Substanzklasse
Tag
Hintergrund
DYKDDDDK tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A DYKDDDDK-Tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a DYKDDDDK-Tag to this protein allows one to follow the protein with an antibody against the DYKDDDDK sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Synonyms: Monoclonal DYKDDDDK antibody, Anti DYKDDDDK antibody, DYKDDDDK antibody, DYKDDDDK antibody, FG4R antibody, DYKDDDDK tag antibody.