PROX1 Antikörper (C-Term)

Produktnummer ABIN929458

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PROX1 in WB, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human und wurde in 3+ Publikationen erwähnt.

Kurzübersicht für PROX1 Antikörper (C-Term) (ABIN929458)

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PROX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PROX1 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody will detect Prox-1 in the nucleus of lymphatic endothelial cells by immunostaining, and human Prox 1 by western blot.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Kreuzreaktivität (Details): Equal reactivity with Human, Mouse, and Rat

Aufreinigung: Protein A affinity chromatography

Immunogen: Prox 1 antibody was raised in rabbit using recombinant human C-terminal part of Prox-1 (homeo domain and prospero domain) as the immunogen

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1-5µg/mL. For other applications, optimal conditions should be determined by end-user
Optimal conditions should be determined by the investigator.

Kommentare:

The lyophilized IgG is stable at 4 °C for at least one month and for more than a year when kept at -20 °C. When reconstituted in sterile water to a concentration of >0.5 mg/mL the antibody is stable for at least six weeks at 2-4 °C in the presence of a preservative.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Lot specific

Buffer: IgG fraction supplied lyophilizedfrom PBS, pH 7.4 with no preservative.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store lyophilized antibody at -20 °C. Following reconstitution store at 4 °C in the short-term. For longer term storage aliquot and store at -20 °C or -70 °C.

Referenzen für anti-PROX1 Antikörper (ABIN929458)

Zhang, Brady, Liang, Wu, Henkemeyer, Yan: "EphB4 forward signalling regulates lymphatic valve development." in: Nature communications, Vol. 6, pp. 6625, (2016) (PubMed).

Kulkarni, Herman, Ikegami, Greenberg, Akeson: "Lymphatic ontogeny and effect of hypoplasia in developing lung." in: Mechanisms of development, Vol. 128, Issue 1-2, pp. 29-40, (2011) (PubMed).

Kulkarni, Greenberg, Akeson: "NFATc1 regulates lymphatic endothelial development." in: Mechanisms of development, Vol. 126, Issue 5-6, pp. 350-65, (2009) (PubMed).

Details zu PROX1

Target: PROX1 (Prospero Homeobox 1 (PROX1))

Andere Bezeichnung: Prox 1

Hintergrund: Prox-1 is a homeobox gene and acts as a master switch for lymphatic endothelial phenotype. Expression of Prox-1 in blood endothelial cells induces expression of other lymphatic marker genes. Together with Podoplanin, Prox-1 can be used to reliably distinguish lympathic vessels from blood vessels. Prox-1 is expressed in CNS, eye, pancreas, liver and heart, and it is one of the most specific and reliable markers for lymphatic endothelial cells. Synonyms: Polyclonal Prox 1 antibody, Anti-Prox 1 antibody, Prox-1 antibody, Prospero related homeobox 1 antibody, Homeobox prospero like protein antibody, Homeobox prospero like protein PROX1 antibody, Prospero homeobox protein 1 antibody, Prospero related homeobox 1 antibody.

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation