AP2A2 Antikörper (C-Term)

Produktnummer ABIN929411

Der Kaninchen Polyklonal Anti-AP2A2-Antikörper wurde für WB validiert. Er ist geeignet, AP2A2 in Proben von Human zu detektieren.

Kurzübersicht für AP2A2 Antikörper (C-Term) (ABIN929411)

Target: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AP2A2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-AP2A2 Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Purified

Immunogen: AP2 A2 antibody was raised in rabbit using the C terminal of AP2 2 as the immunogen

Anwendungsinformationen

Applikationshinweise: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Kommentare:

AP2A2 Blocking Peptide, (ABIN5612096), is also available for use as a blocking control in assays to test for specificity of this AP2A2 antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Details zu AP2A2

Target: AP2A2 (Adaptor-Related Protein Complex 2, alpha 2 Subunit (AP2A2))

Andere Bezeichnung: AP2A2

Hintergrund: AP2A2 is a component of the adaptor protein complex 2 (AP-2). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. Synonyms: Polyclonal AP2A2 antibody, Anti-AP2A2 antibody, adaptor-related protein complex 2, alpha 2 subunit antibody, ADTAB antibody, CLAPA2 antibody, HIP9 antibody, HYPJ antibody.

Pathways: EGFR Signaling Pathway, Neurotrophin Signalübertragung, EGFR Downregulation, SARS-CoV-2 Protein Interaktom