His Tag Antikörper (HRP)

Produktnummer ABIN920804

Der Huhn Polyklonal Anti-His Tag-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, His Tag in Proben von zu detektieren.

Kurzübersicht für His Tag Antikörper (HRP) (ABIN920804)

Target: His Tag

Reaktivität: Bitte anfragen

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser His Tag Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-His Tag Antikörper (HRP)

Sequenz: HHHHHH

Anwendungsinformationen

Applikationshinweise: Western blot: Use at 1:1,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 50 mM HEPES pH 7.0, 0.1M Sodium Chloride with 0.2% BSA and 0.01% thimerosal.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu His Tag

Target: His Tag

Andere Bezeichnung: Hexahistidine-Tag

Substanzklasse: Tag

Hintergrund: Chickens were immunized with HHHHHH peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity- purified using the peptide immobilized on a solid support and the antibodies were conjugated with horseradish peroxidase (HRP). Specification: ELISA: HRP-conjugated chicken anti- hexahistidine diluted 1:10,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti-peptide purification.